The effect of tuning cold plasma composition on glioblastoma cell viability.
The effect of tuning cold plasma composition on glioblastoma cell viability.
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DOI：
10.1371/journal.pone.0098652
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Keidar M
中科院分区：
文献类型：
--
作者：
Cheng X;Sherman J;Murphy W;Ratovitski E;Canady J;Keidar M
先前在冷大气等离子体（CAP）和癌细胞相互作用方面的研究已经多次证明冷等离子体诱导细胞死亡。据推测，活性氧（ROS）和活性氮（RNS）在CAP癌症治疗中起主要作用。在本文中，我们试图确定CAP治疗胶质母细胞瘤细胞（U87）的机制，通过了解等离子体的组成，包括治疗时间，电压，流量和等离子体气体组成。为了确定对U87的等离子体处理的阈值，使用正常人星形胶质细胞（E6/E7）作为比较细胞系。我们的数据显示，与E6/E7细胞相比，30秒等离子体处理在U87细胞中引起3倍的细胞死亡。冷等离子体的所有其他组成基于该结果进行：等离子体处理时间保持在30秒/孔，而其他等离子体特性如电压、源气体的流速和源气体的组成一次一个地改变以改变等离子体射流中的反应性物质组成的强度，这最终可能对细胞具有由细胞活力反映的各种影响。我们定义了一个术语“血浆剂量”来总结所有特征和细胞活力的关系。
Previous research in cold atmospheric plasma (CAP) and cancer cell interaction has repeatedly proven that the cold plasma induced cell death. It is postulated that the reactive oxygen species (ROS) and reactive nitrogen species (RNS) play a major role in the CAP cancer therapy. In this paper, we seek to determine a mechanism of CAP therapy on glioblastoma cells (U87) through an understanding of the composition of the plasma, including treatment time, voltage, flow-rate and plasma-gas composition. In order to determine the threshold of plasma treatment on U87, normal human astrocytes (E6/E7) were used as the comparison cell line. Our data showed that the 30 sec plasma treatment caused 3-fold cell death in the U87 cells compared to the E6/E7 cells. All the other compositions of cold plasma were performed based on this result: plasma treatment time was maintained at 30 s per well while other plasma characteristics such as voltage, flow rate of source gas, and composition of source gas were changed one at a time to vary the intensity of the reactive species composition in the plasma jet, which may finally have various effect on cells reflected by cell viability. We defined a term “plasma dosage” to summarize the relationship of all the characteristics and cell viability.
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