Transcriptomic and GC-MS Metabolomic Analyses Reveal the Sink Strength Changes during Petunia Anther Development.
Transcriptomic and GC-MS Metabolomic Analyses Reveal the Sink Strength Changes during Petunia Anther Development.
复制标题
转录组学和 GC-MS 代谢组学分析揭示矮牵牛花药发育过程中库强度的变化
DOI：
10.3390/ijms19040955
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发表时间：
2018-03-23
影响因子：
5.6
通讯作者：
Hu H
中科院分区：
文献类型：
--
作者：
Yue Y;Tian S;Wang Y;Ma H;Liu S;Wang Y;Hu H
矮牵牛是一种具有较高观赏价值的双子叶草本花卉，在园林绿化中已被广泛栽培。每年，世界范围内对矮牵牛种子有巨大的市场需求。花药的正常发育是植物产生种子的必要前提。然而，对矮牵牛花药发育过程的认识仍然有限。为了更好地了解矮牵牛花药发育的机制，构建了矮牵牛花药发育3个典型时期的转录组和代谢组，并利用它们检测了矮牵牛花药发育过程中基因表达模式和初级代谢产物的变化。结果表明，在花药发育的不同阶段，存在着许多差异表达基因，主要参与光合作用和淀粉、蔗糖代谢。本研究采用偏最小二乘判别分析（PLS-DA）方法，将参与淀粉和蔗糖代谢的果糖和葡萄糖作为最重要的代谢产物。此外，qRT-PCR分析表明，随着花药发育，光合相关基因的表达量均呈现沿着降低的趋势。这些证据表明，在花药发育的各个阶段，能量和碳水化合物代谢途径的活性逐渐降低，从而影响了库的强度。总的来说，这项工作提供了一个新的和全面的了解矮牵牛花药的代谢过程。
Petunia, which has been prevalently cultivated in landscaping, is a dicotyledonous herbaceous flower of high ornamental value. Annually, there is a massive worldwide market demand for petunia seeds. The normal development of anther is the necessary prerequisite for the plants to generate seeds. However, the knowledge of petunia anther development processes is still limited. To better understand the mechanisms of petunia anther development, the transcriptomes and metabolomes of petunia anthers at three typical development stages were constructed and then used to detect the gene expression patterns and primary metabolite profiles during the anther development processes. Results suggested that there were many differentially-expressed genes (DEGs) that mainly participated in photosynthesis and starch and sucrose metabolism when DEGs were compared between the different development stages of anthers. In this study, fructose and glucose, which were involved in starch and sucrose metabolism, were taken as the most important metabolites by partial least-squares discriminate analysis (PLS-DA). Additionally, the qRT-PCR analysis of the photosynthetic-related genes all showed decreased expression trends along with the anther development. These pieces of evidence indicated that the activities of energy and carbohydrate metabolic pathways were gradually reduced during all the development stages of anther, which affects the sink strength. Overall, this work provides a novel and comprehensive understanding of the metabolic processes in petunia anthers.
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影响因子：
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作者：
Bokvaj P;Hafidh S;Honys D
通讯作者：
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影响因子：
7.4
作者：
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通讯作者：
Kuhlemeier, Cris
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通讯作者：
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