Airway surface mycosis in chronic TH2-associated airway disease.
Airway surface mycosis in chronic TH2-associated airway disease.
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DOI：
10.1016/j.jaci.2014.04.028
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发表时间：
2014-08
影响因子：
14.2
通讯作者：
Luong, Amber
中科院分区：
文献类型：
--
作者：
Porter, Paul C.;Lim, Dae Jun;Maskatia, Zahida Khan;Mak, Garbo;Tsai, Chu-Lin;Citardi, Martin J.;Fakhri, Samer;Shaw, Joanne L.;Fothergil, Annette;Kheradmand, Farrah;Corry, David B.;Luong, Amber
环境真菌与辅助性T型2 （Th2）细胞相关的气道炎症和Th2相关的慢性气道疾病哮喘、慢性鼻鼻窦炎伴鼻息肉（CRSwNP）和变应性真菌鼻鼻窦炎（AFRS）有关，但这些微生物是否直接或间接参与疾病病理尚不清楚。目的：测定th2相关和非相关气道疾病患者的真菌分离频率和真菌特异性免疫。收集鼻窦手术患者（118例）鼻窦灌洗液和血液，包括伴有和不伴有鼻息肉、AFRS的CRS患者以及非CRS/非哮喘对照患者。根据病史确定哮喘状况。培养鼻窦灌洗液并直接检查是否有活菌存在。用酶联免疫细胞斑点法（ELISpot）检测真菌抗原刺激外周血单个核细胞，以测定总记忆真菌特异性il -4分泌细胞。将这些数据与ELISA从血浆中测量的真菌特异性IgE水平进行比较。与非th2相关的对照患者（n=31）相比，th2相关气道疾病患者（哮喘、CRSwNP或AFRS: n=68）培养的丝状真菌明显更常见；分别为74% vs 16%， p<0.001。真菌特异性IL-4 ELISpot （n=48）和特异性IgE （n=70）数据均与th2相关疾病相关（敏感性73%，特异性100%，分别为50%和77%）。仅在th2相关的慢性气道疾病患者中，经常分离到直接生长在气道内并伴有对这些生物体的特异性免疫的真菌，这表明真菌直接参与了这些疾病的发病机制。应考虑在选定的患者中努力根除气道真菌。气道真菌可能有助于鼻窦炎伴鼻息肉和哮喘的表达，提示应考虑从这些患者的气道和环境中根除真菌。
Environmental fungi have been linked to T helper type 2 (Th2) cell-related airway inflammation and the Th2-associated chronic airway diseases asthma, chronic rhinosinusitis with nasal polyps (CRSwNP) and allergic fungal rhinosinusitis (AFRS), but whether these organisms participate directly or indirectly in disease pathology remains unknown. To determine the frequency of fungus isolation and fungus-specific immunity in Th2-associated and non-associated airway disease patients. Sinus lavage fluid and blood were collected from sinus surgery patients (n=118) including CRS patients with and without nasal polyps and AFRS and non-CRS/non-asthmatic control patients. Asthma status was deteremined from medical history. Sinus lavage fluids were cultured and directly examined for evidence of viable fungi. Peripheral blood mononuclear cells were restimulated with fungal antigens in an enzyme linked immunocell spot (ELISpot) assay to determine total memory fungus-specific IL-4-secreting cells. These data were compared to fungus-specific IgE levels measured from plasma by ELISA. Filamentous fungi were significantly more commonly cultured from Th2-associated airway disease subjects (asthma, CRSwNP, or AFRS: n=68) compared to non-Th2-associated control patients (n=31); 74% vs 16% respectively, p<0.001. Both fungus-specific IL-4 ELISpot (n=48) and specific IgE (n=70) data correlated with Th2-associated diseases (sensitivity 73% and specificity 100% vs. 50% and 77%, respectively). The frequent isolation of fungi growing directly within the airways accompanied by specific immunity to these organisms only in patients with Th2-associated chronic airway diseases suggests that fungi participate directly in the pathogenesis of these conditions. Efforts to eradicate airway fungi from the airways should be considered in selected patients. Airway fungi may contribute to the expression of sinusitis with nasal polyps and asthma, suggesting that efforts to eradicate fungi from the airways and environments of these patients should be considered.
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DOI：
10.1126/science.1240342
发表时间：
2013-08-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Millien VO;Lu W;Shaw J;Yuan X;Mak G;Roberts L;Song LZ;Knight JM;Creighton CJ;Luong A;Kheradmand F;Corry DB
通讯作者：
Corry DB
影响因子：
10
作者：
Marks, GB;Colquhoun, JR;Car, NG
通讯作者：
Car, NG
影响因子：
14.2
作者：
Pulimood, Thomas B.;Corden, Julie M.;Nasser, Shuaib M.
通讯作者：
Nasser, Shuaib M.
DOI：
10.1164/rccm.200805-737oc
发表时间：
2009-01-01
影响因子：
24.7
作者：
Denning, David W.;O'Driscoll, B. Ronan;Niven, Robert M.
通讯作者：
Niven, Robert M.
影响因子：
1.9
作者：
Chishimba, Livingstone;Niven, Robert M.;Denning, David W.
通讯作者：
Denning, David W.