Fine mapping of the major gene BhHLS1 controlling seed size in wax gourd (Benincasa hispida).
Fine mapping of the major gene BhHLS1 controlling seed size in wax gourd (Benincasa hispida).
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主要基因BHHLS1的精细映射控制蜡葫芦（Benincasa hispida）中的种子大小。
DOI：
10.3389/fpls.2023.1266796
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发表时间：
2023
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
冬瓜籽粒大小是一个重要的农艺性状，但其相关基因尚未见报道。本研究利用由MY-1(大粒)和GX-71(小粒)品系杂交产生的F8重组自交系群体构建的高密度遗传图谱，对冬瓜籽粒大小相关性状的数量性状基因座(QTL)进行了为期两年的检测。连续两年(2021-2022)重复检测到10号染色体上2个稳定的QTL(qSL10和qSW10)，分别控制籽粒长度(SL)和籽粒宽度(Sw)。2021年和2022年，qSL10的表型变异率分别为75.30%和80.80%。而qSW10在2021年和2022年的表型变异率分别为66.60%和73.80%。此外，通过对候选基因的测序分析发现，单核苷酸多态突变导致GX-71中Bch10G006400(BhHLS1)翻译的提前终止。根据基因功能注释和实时定量聚合酶链式反应分析，BhHLS1编码一个可能的N-乙酰转移酶HLS1蛋白，其表达水平在亲本之间存在显著差异。因此，BhHLS1是一个与调节冬瓜SL和SW的单因素多态性相关的主要候选基因。最后，基于BhHLS1序列的变异，开发了一种裂解扩增多态序列标记，用于冬瓜的分子标记辅助育种。总之，本研究对冬瓜种子大小的遗传改良、基因功能的验证以及特定种质资源的培育具有重要意义。
The seed size of wax gourds is an important agronomic trait; however, the associated genes have not yet been reported. In this study, we used a high-density genetic map constructed based on F8 recombinant inbred line populations derived from a cross between MY-1 (large seed) and GX-71 (small seed) strains to detect quantitative trait locis (QTLs) for seed-size-related traits in wax gourd over a two-year period. Two stable QTLs (qSL10 and qSW10) for seed length (SL) and seed width (SW) on chromosome 10 were repeatedly detected over two years (2021–2022). qSL10 had a phenotypic variation rate of 75.30% and 80.80% in 2021 and 2022, respectively. Whereas, qSW10 had a phenotypic variation rate of 66.60% and 73.80% in 2021 and 2022, respectively. Further, a single nucleotide polymorphism mutation was found to cause early termination of Bch10G006400 (BhHLS1) translation in GX-71 through sequencing analysis of candidate genes. Based on gene functional annotation and quantitative real-time PCR analyses, BhHLS1 encoded a probable N-acetyltransferase HLS1-like protein and its expression level was significantly different between parents. Therefore, BhHLS1 is a major candidate gene associated with a one-factor polymorphism regulating the SL and SW of wax gourds. Finally, based on variation in the BhHLS1 sequence, a cleaved amplified polymorphic sequence marker was developed for the molecular marker-assisted breeding of wax gourds. Overall, this study is of great significance for the genetic improvement of seed size, verification of gene functions, and cultivation of specific germplasm resources for wax gourds.
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影响因子：
5.6
作者：
Ma L;Liu Z;Cheng Z;Gou J;Chen J;Yu W;Wang P
通讯作者：
Wang P
影响因子：
1.7
作者：
Martinez-Lopez, Marcela;Tinoco-Ojanguren, Clara;Martorell, Carlos
通讯作者：
Martorell, Carlos
影响因子：
5.6
作者：
通讯作者：
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影响因子：
3.5
作者：
通讯作者：
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影响因子：
27.5
作者：
Hu, Zejun;Lu, Sun-Jie;Liu, Jian-Xiang
通讯作者：
Liu, Jian-Xiang