Identification of the Key Pathways and Genes Involved in the Wax Biosynthesis of the Chinese White Wax Scale Insect (Ericerus pela Chavannes) by Integrated Weighted Gene Coexpression Network Analysis.
Identification of the Key Pathways and Genes Involved in the Wax Biosynthesis of the Chinese White Wax Scale Insect (Ericerus pela Chavannes) by Integrated Weighted Gene Coexpression Network Analysis.
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中国白色蜡蚧（CWWS）雄虫分泌的白色蜡是一种具有重要经济价值的天然高分子化合物。但其对蜡生物合成的调控机制尚不清楚。在这项研究中，加权基因共表达网络分析（WGCNA）被用来分析转录组数据的第一和第二龄的女性，早期和晚期的女性成年人，和第一和第二龄的男性。共获得19个拓扑重叠度不同的模块，其中3个模块与泌蜡量显著相关（p < 0.05）。通过筛选共获得30个枢纽基因，其中超长链脂肪酸延伸蛋白（ExtensioningofVery-LongChainFatyAcidsProtein，EFL）和脂肪酰辅酶A还原酶（FatyAcyl-CoA Reductase，FAR）是重要的脂肪酸代谢催化酶。此外，它们的代谢催化产物参与蜡的生物合成。结果表明，WGCNA可用于昆虫转录组分析，有效筛选蜡质生物合成相关的关键基因。
The white wax secreted by the male insects of the Chinese white wax scale (CWWS) is a natural high-molecular-weight compound with important economic value. However, its regulatory mechanism of wax biosynthesis is still unclear. In this study, a weighted gene coexpression network analysis (WGCNA) was used to analyze transcriptome data of first- and second-instar females, early and late female adults, and first- and second-instar males. A total of 19 partitioned modules with different topological overlaps were obtained, and three modules were identified as highly significant for wax secretion (p < 0.05). A total of 30 hub genes were obtained through screening, among which elongation of very-long-chain fatty acids protein (ELOVL) and fatty acyl-CoA reductase (FAR) are important catalytic enzymes of fatty acid metabolism. Furthermore, their metabolic catalytic products are involved in the synthesis of wax biosynthesis. The results demonstrate that WGCNA can be used for insect transcriptome analysis and effectively screen out the key genes related to wax biosynthesis.
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WGCNA：用于加权相关网络分析的 R 包。
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