Antibody-dependent antitumor cytotoxicity by human monocytes cultured with recombinant macrophage colony-stimulating factor. Induction of efficient antibody-mediated antitumor cytotoxicity not detected by isotope release assays.
Antibody-dependent antitumor cytotoxicity by human monocytes cultured with recombinant macrophage colony-stimulating factor. Induction of efficient antibody-mediated antitumor cytotoxicity not detected by isotope release assays.
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用重组巨噬细胞刺激因子培养的人类单核细胞的抗体依赖性抗肿瘤细胞毒性。同位素释放分析未检测到有效抗体介导的抗体细胞毒性。
DOI：
10.1084/jem.170.2.511
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发表时间：
1989-08-01
影响因子：
15.3
通讯作者：
CHEUNG, NKV
中科院分区：
文献类型：
--
作者：
MUNN, DH;CHEUNG, NKV
巨噬细胞集落刺激因子（M-CSF）是已知的刺激单核细胞/巨噬细胞祖细胞的增殖和增强体外抗肿瘤细胞毒性的鼠巨噬细胞。在本文中，我们已经表明，重组人M-CSF导致人外周血单核细胞在文化中分化成代谢活性的巨噬细胞样细胞。在两种鼠IgG 3 mAb（3F 8和R24）存在下，这些细胞介导针对人黑素瘤和神经母细胞瘤细胞系的非常有效的抗体依赖性细胞毒性（ADCC）。它们还在较小程度上介导抗体非依赖性细胞毒性（或细胞抑制）。人血清对ADCC的影响不一致，但通常诱导相似的高水平ADCC。使用新的ELISA检测ADCC后存活的肿瘤细胞来测量细胞毒性。两种传统的同位素释放试验（51 Cr和[3 H]TdR）低估或完全无法检测到M-CSF激活的单核细胞的ADCC。最佳激活发生在100-300 U/ml的M-CSF，并需要9-11 d完成。大多数M-CSF培养的单核细胞表达低亲和力Fc受体（CD 16）。单核细胞/巨噬细胞系细胞使用鼠IgG 3 mAb的ADCC可能对人类恶性肿瘤的免疫治疗具有重要意义。
Macrophage colony-stimulating factor (M-CSF) is known to stimulate proliferation of monocyte/macrophage progenitors and enhance in vitro antitumor cytotoxicity by murine macrophages. In this paper we have shown that recombinant human M-CSF causes human peripheral blood monocytes to differentiate in culture into metabolically active macrophage-like cells. These cells mediate very efficient antibody- dependent cellular cytotoxicity (ADCC) against human melanoma and neuroblastoma cell lines in the presence of two murine IgG3 mAbs (3F8 and R24). They also mediate antibody-independent cytotoxicity (or cytostasis) to a lesser extent. Human serum had an inconsistent effect on ADCC, but often induced similar high levels of ADCC. Cytotoxicity was measured using a novel ELISA to detect surviving tumor cells after ADCC. Two conventional isotope-release assays (51Cr and [3H]TdR) underestimated or entirely failed to detect ADCC by M-CSF-activated monocytes. Optimal activation occurred with 100-300 U/ml of M-CSF, and required 9-11 d for completion. Most of the M-CSF cultured monocytes expressed the low-affinity Fc receptor (CD16). ADCC by cells of the monocyte/macrophage lineage using murine IgG3 mAbs may have significance for the immunotherapy of human malignancies.
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DOI：
10.1073/pnas.81.11.3506
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
ADAMS, DO;HALL, T;KOPROWSKI, H
通讯作者：
KOPROWSKI, H
影响因子：
4.3
作者：
AMPEL, NM;WING, EJ;SHADDUCK, RK
通讯作者：
SHADDUCK, RK
影响因子：
9.7
作者：
GAUCI, CL;ALEXANDER, P
通讯作者：
ALEXANDER, P
影响因子：
56.9
作者：
GRABSTEIN, KH;URDAL, DL;CONLON, PJ
通讯作者：
CONLON, PJ
影响因子：
2.7
作者：
CALAFAT, J;JANSSEN, H;HEKMAN, A
通讯作者：
HEKMAN, A