Role of polo kinase and Mid1p in determining the site of cell division in fission yeast.
Role of polo kinase and Mid1p in determining the site of cell division in fission yeast.
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DOI：
10.1083/jcb.143.6.1603
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发表时间：
1998-12-14
期刊：
影响因子：
--
通讯作者：
McCollum D
中科院分区：
文献类型：
--
作者：
Bähler J;Steever AB;Wheatley S;Wang Yl;Pringle JR;Gould KL;McCollum D
分裂酵母裂糖酵母（Schizosaccharomyces pombe）利用中间的f -肌动蛋白为基础的收缩环进行对称分裂，产生大小相等的子细胞。先前描述的两个基因，mid1和pom1的突变缺陷，经常不对称分裂。在这里，我们提出了三个新的温度敏感突变体在分裂面定位缺陷的鉴定。这三种突变体在polo激酶基因plo1中都有突变，并且在中间环的位置和组织上都表现出与mid1突变体非常相似的缺陷。在这两种情况下，环的形成经常在细胞极点附近开始，这表明Mid1p和Plo1p在将内侧环成分招募到细胞中心方面起作用。先前有报道称，在有丝分裂过程中，Mid1p过度磷酸化，并从细胞核迁移到内环。我们发现，在纺锤体形成过程中，Mid1p首先形成弥漫性皮质带，然后在后期形成环状。Plo1p是Mid1p离开细胞核形成环所必需的，Pom1p是Mid1p环正确放置所必需的。当Plo1p过表达时，Mid1p提前退出细胞核，并在凝胶上表现出与先前在有丝分裂细胞中观察到的过度磷酸化形式相似的迁移性降低。遗传和双杂交分析表明，Plo1p和Mid1p在一个与Pom1p不同的共同途径中起作用。Plo1p定位于有丝分裂细胞的纺锤极体和纺锤体，也定位于有丝分裂细胞形成时的内侧环。综上所述，这些数据表明Plo1p通过调节Mid1p在分裂位点定位中发挥作用。鉴于其先前已知的在有丝分裂和细胞质分裂时间中的功能，Plo1p因此被认为是细胞质分裂与有丝分裂的空间和时间协调的关键分子。
The fission yeast Schizosaccharomyces pombe divides symmetrically using a medial F-actin– based contractile ring to produce equal-sized daughter cells. Mutants defective in two previously described genes, mid1 and pom1, frequently divide asymmetrically. Here we present the identification of three new temperature-sensitive mutants defective in localization of the division plane. All three mutants have mutations in the polo kinase gene, plo1, and show defects very similar to those of mid1 mutants in both the placement and organization of the medial ring. In both cases, ring formation is frequently initiated near the cell poles, indicating that Mid1p and Plo1p function in recruiting medial ring components to the cell center. It has been reported previously that during mitosis Mid1p becomes hyperphosphorylated and relocates from the nucleus to a medial ring. Here we show that Mid1p first forms a diffuse cortical band during spindle formation and then coalesces into a ring before anaphase. Plo1p is required for Mid1p to exit the nucleus and form a ring, and Pom1p is required for proper placement of the Mid1p ring. Upon overexpression of Plo1p, Mid1p exits the nucleus prematurely and displays a reduced mobility on gels similar to that of the hyperphosphorylated form observed previously in mitotic cells. Genetic and two-hybrid analyses suggest that Plo1p and Mid1p act in a common pathway distinct from that involving Pom1p. Plo1p localizes to the spindle pole bodies and spindles of mitotic cells and also to the medial ring at the time of its formation. Taken together, the data indicate that Plo1p plays a role in the positioning of division sites by regulating Mid1p. Given its previously known functions in mitosis and the timing of cytokinesis, Plo1p is thus implicated as a key molecule in the spatial and temporal coordination of cytokinesis with mitosis.
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DOI：
10.1083/jcb.129.6.1617
发表时间：
1995-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Golsteyn RM;Mundt KE;Fry AM;Nigg EA
通讯作者：
Nigg EA
影响因子：
64.5
作者：
GYURIS, J;GOLEMIS, E;BRENT, R
通讯作者：
BRENT, R
影响因子：
0.9
作者：
Edamatsu, M;Toyoshima, YY
通讯作者：
Toyoshima, YY
影响因子：
3.3
作者：
Ding, R;West, RR;McIntosh, JR
通讯作者：
McIntosh, JR
影响因子：
10.5
作者：
CVRCKOVA, F;DEVIRGILIO, C;NASMYTH, K
通讯作者：
NASMYTH, K