Tailoring fungal morphology of Aspergillus niger MYA 135 by altering the hyphal morphology and the conidia adhesion capacity: biotechnological applications.
Tailoring fungal morphology of Aspergillus niger MYA 135 by altering the hyphal morphology and the conidia adhesion capacity: biotechnological applications.
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DOI：
10.1186/2191-0855-3-27
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发表时间：
2013-05-20
期刊：
影响因子：
3.7
通讯作者：
Pera LM
中科院分区：
文献类型：
--
作者：
Colin VL;Baigorí MD;Pera LM
丝状真菌发酵及其作为微生物细胞工厂的进一步成功发展目前存在的问题取决于控制真菌形态。在这方面，这项工作探索了新的实验程序，以定量检查的潜力，一些培养条件，以诱导确定的真菌形态，通过改变菌丝形态和分生孢子的粘附能力。通过研究一些培养条件对尼日尔MYA 135细胞壁裂解潜力的影响，评价了环境条件改变菌丝形态的能力。通过测量细胞壁裂解酶活性如菌丝体结合的β-N-乙酰基-D-氨基葡糖苷酶（Mb-NAGase）来确定细胞壁裂解潜力的相对值。另一方面，分生孢子粘附力的定量值被认为是其聚集能力的指标。在显微形态上，菌丝生长单位长度（lHGU）与Mb NAG酶活性呈极显著负相关（r =-0.915，P < 0.001）。事实上，只有在与等于或高于190 U gdry.wt-1的特定Mb-NAG酶值相容的那些培养条件下，环境才能够诱导高度分支的菌丝体。就宏观形态而言，分生孢子粘附能力较低，随后是分散的菌丝生长。事实上，该研究表明，每毫升等于或高于0.50的分生孢子粘附单位是形成沉淀所必需的。此外，还观察到，一旦形成粒料，IHGU对其最终直径具有重要影响。最后讨论了这些结果的生物技术意义。
Current problems of filamentous fungi fermentations and their further successful developments as microbial cell factories are dependent on control fungal morphology. In this connection, this work explored new experimental procedures in order to quantitatively check the potential of some culture conditions to induce a determined fungal morphology by altering both hyphal morphology and conidia adhesion capacity. The capacity of environmental conditions to modify hyphal morphology was evaluated by examining the influence of some culture conditions on the cell wall lytic potential of Aspergillus niger MYA 135. The relative value of the cell wall lytic potential was determined by measuring a cell wall lytic enzyme activity such as the mycelium-bound β-N-acetyl-D-glucosaminidase (Mb-NAGase). On the other hand, the quantitative value of conidia adhesion was considered as an index of its aggregation capacity. Concerning microscopic morphology, a highly negative correlation between the hyphal growth unit length (lHGU) and the specific Mb-NAGase activity was found (r = -0.915, P < 0.001). In fact, the environment was able to induce highly branched mycelia only under those culture conditions compatible with specific Mb-NAGase values equal to or higher than 190 U gdry.wt-1. Concerning macroscopic morphology, a low conidia adhesion capacity was followed by a dispersed mycelial growth. In fact, this study showed that conidia adhesion units per ml equal to or higher than 0.50 were necessary to afford pellets formation. In addition, it was also observed that once the pellet was formed the lHGU had an important influence on its final diameter. Finally, the biotechnological significance of such results was discussed as well.
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影响因子：
--
作者：
Newey, Lisa J.;Caten, Chris E.;Green, Jonathan R.
通讯作者：
Green, Jonathan R.
影响因子：
2.1
作者：
Pera, LM;Rubinstein, L;Callieri, DA
通讯作者：
Callieri, DA
影响因子：
3.1
作者：
Leticia Colin, Veronica;Domingo Baigori, Mario;Maria Pera, Licia
通讯作者：
Maria Pera, Licia
影响因子：
2.8
作者：
Ichinomiya, M;Motoyama, T;Ohta, A
通讯作者：
Ohta, A
影响因子：
4.4
作者：
El-Enshasy, Hesham;Kleine, Joachim;Rinas, Ursula
通讯作者：
Rinas, Ursula