Noncontact, Laser-Mediated Extraction of Polar Bodies for Prefertilization Genetic Diagnosis
Noncontact, Laser-Mediated Extraction of Polar Bodies for Prefertilization Genetic Diagnosis
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非接触式激光介导的极体提取用于受精前遗传诊断
DOI：
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发表时间：
2002
影响因子：
3.1
通讯作者：
F. Berg
中科院分区：
文献类型：
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作者：
Annette Clement;T. Buchholz;K. Schütze;U. Berg;F. Berg
目的：我们测试了一种完全非接触式极体提取方法，使用用于激光带钻孔的紫外激光束和用于捕获和提取极体 (PB) 的近红外激光束。方法：在卵母细胞的透明带上钻一个孔。然后，用光镊夹住 PB 并将其拖过钻孔。结果：使用该方法可以从 63 个卵母细胞中的 49 个（78%）中提取出牛的第一 PB。在人类卵母细胞中，PB 提取已被成功证明，但比较耗时。一些提取的 PB 在特殊膜上干燥，用激光微束进行包皮环切，并成功弹射到微量离心管的盖子中（激光压力弹射）。结论：这种仅激光介导的提取方法可以方便地采购 PB，而没有污染的危险，并且是一种有前景的方法，将来可能会取代标准的显微操作方法。
Purpose: We tested an entirely noncontact polar body-extraction method using an ultraviolet laser beam for laser zona drilling and a near infrared laser beam for polar body (PB) trapping and extraction. Methods: A hole was drilled into the zona pellucida of an oocyte. Then, the PB was trapped with optical tweezers and dragged through the drilled hole. Results: Bovine first PBs could be extracted in 49 out of 63 oocytes (78%) using this method. In human oocytes, PB extraction was successfully demonstrated, which however was more time consuming. A number of extracted PBs were dried on a special membrane, circumcised with the laser microbeam, and successfully catapulted into the lid of a microfuge tube (laser pressure catapulting). Conclusions: This solely laser-mediated extraction method allows convenient procurement of PBs without the danger of contamination and is a promising approach that might replace standard micromanipulation methods in the future.
人类精子产生的力与速度相关，并使用激光产生的光陷阱确定。
影响因子：
6.7
作者：
Tadir,Y;Wright,WH;Vafa,O;Ord,T;Asch,RH;Berns,MW
通讯作者：
Berns,MW
使用红外激光光阱在三个维度上控制人类精子的显微操作：对精子速度的影响。
影响因子：
6.7
作者：
Colon,JM;Sarosi,P;McGovern,PG;Askin,A;Dziedzic,JM;Skurnick,J;Weiss,G;Bonder,EM
通讯作者：
Bonder,EM