Large scale production and characterization of SARS-CoV-2 whole antigen for serological test development.
Large scale production and characterization of SARS-CoV-2 whole antigen for serological test development.
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DOI：
10.1002/jcla.23735
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发表时间：
2021-04
影响因子：
2.7
通讯作者：
Brogi A
中科院分区：
文献类型：
--
作者：
Cerutti H;Ricci V;Tesi G;Soldatini C;Castria M;Vaccaro MN;Tornesi S;Toppi S;Verdiani S;Brogi A
严重急性呼吸系统综合征冠状病毒2（SARS-CoV-2）的快速传播已经在全球范围内产生了一场死亡率惊人的大流行。自2019年冠状病毒病（COVID-19）确诊以来，这种情况对试图满足对诊断工具的迫切需求的临床实验室产生了重大影响。必须开发一种可靠的血清学诊断免疫测定法，具有高水平的灵敏度和特异性，以检测SARS-CoV-2抗体，并改善与其他循环病毒的鉴别诊断。开发了一种使用体外培养的全灭活病毒的酶联免疫吸附试验（ELISA），用于检测病毒抗原。用WB和ELISA法对恢复期病人血清和阴性血清进行了检测。同时使用重组MAB进行两项分析，以验证结果。使用这种免疫测定法获得的10份血清（5名COVID-19患者和5名健康对照）的初步数据非常有希望，成功识别了所有确诊的SARS-CoV-2阳性个体。这种ELISA似乎是检测COVID-19抗体（IgG，IgM和伊加）的特异性和可靠的方法，也是识别对病毒产生免疫力的个体的有用工具。严重急性呼吸系统综合征冠状病毒2（SARS-CoV-2）的快速传播已经在全球范围内产生了一场死亡率惊人的大流行。这种ELISA似乎是检测COVID-19抗体（IgG，IgM和伊加）的特异性和可靠的方法，并为识别已对病毒产生免疫力的个体提供了有用的工具。
The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) has generated a pandemic with alarming rates of fatality worldwide. This situation has had a major impact on clinical laboratories that have attempted to answer the urgent need for diagnostic tools, since the identification of coronavirus disease 2019 (COVID‐19). Development of a reliable serological diagnostic immunoassay, with high levels of sensitivity and specificity to detect SARS‐CoV‐2 antibodies with improved differential diagnosis from other circulating viruses, is mandatory. An enzyme‐linked immunosorbent assay (ELISA) using whole inactivated virus cultured in vitro, was developed to detect viral antigens. WB and ELISA investigations were carried out with sera of convalescent patients and negative sera samples. Both analyses were concurrently performed with recombinant MABs to verify the findings. Preliminary data from 10 sera (5 patients with COVID‐19, and 5 healthy controls) using this immunoassay are very promising, successfully identifying all of the confirmed SARS‐CoV‐2‐positive individuals. This ELISA appears to be a specific and reliable method for detecting COVID‐19 antibodies (IgG, IgM, and IgA), and a useful tool for identifying individuals which have developed immunity to the virus. The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) has generated a pandemic with alarming rates of fatality worldwide. This ELISA appears to be a specific and reliable method for detecting COVID‐19 antibodies (IgG, IgM and IgA), and provides a useful tool for identifying individuals which have developed immunity to the virus.
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DOI：
10.1038/nrmicro2147
发表时间：
2009-06
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
2.2
作者：
Chowdhury P;Topno R;Khan SA;Mahanta J
通讯作者：
Mahanta J
DOI：
10.1001/jama.2020.7869
发表时间：
2020-06-09
期刊：
JAMA
影响因子：
--
作者：
Kirkcaldy RD;King BA;Brooks JT
通讯作者：
Brooks JT
影响因子：
158.5
作者：
Gudbjartsson, Daniel F.;Norddahl, Gudmundur L.;Stefansson, Kari
通讯作者：
Stefansson, Kari
影响因子：
13.2
作者：
Zhang, Wei;Du, Rong-Hui;Zhou, Peng
通讯作者：
Zhou, Peng