Recent transfer of an iron-regulated gene from the plastid to the nuclear genome in an oceanic diatom adapted to chronic iron limitation.
Recent transfer of an iron-regulated gene from the plastid to the nuclear genome in an oceanic diatom adapted to chronic iron limitation.
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DOI：
10.1186/1471-2164-11-718
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发表时间：
2010-12-20
期刊：
影响因子：
4.4
通讯作者：
LaRoche J
中科院分区：
文献类型：
--
作者：
Lommer M;Roy AS;Schilhabel M;Schreiber S;Rosenstiel P;LaRoche J
虽然铁限制在当代海洋中的重要性和广泛发生是有据可查的，但我们对浮游植物对这些环境的遗传适应仍然知之甚少。海洋硅藻Thalassiosira oceanica相对于其沿海亲缘物种Thalassiosira dogana而言，对铁限制的耐受性很高。T. oceanica的死亡归因于富含铁的光合作用成分的减少。T.海洋生物可能揭示了这两个物种之间生理差异的遗传基础。该基因全长141790 bp，为T.通过大规模平行焦磷酸测序（454）鸟枪法读取组装的oceanica叶绿体基因组[GenBank：GU323224]显示，编码铁氧还蛋白的petF基因定位于T.在T.海洋。铁-硫蛋白铁氧还蛋白是叶绿体电子传递链的关键元件，在铁限制生长条件下，铁-硫蛋白铁氧还蛋白可以被无铁黄素还蛋白所取代，从而有助于减少细胞对铁的需求。通过与T. taganapetF基因，T. oceanica直向同源物可以追溯到其叶绿体起源。T. oceanica叶绿体基因组与T.虽然一个新的表达的ORF出现在基因组区域中，该区域已经经历了与petF基因转移事件相关的重排。将petF从T. oceanica代表了这两个密切相关的物种之间的主要区别。T. oceanica能够耐受铁限制表明petF从叶绿体转移到核基因组可能有助于该物种的生态成功。
Although the importance and widespread occurrence of iron limitation in the contemporary ocean is well documented, we still know relatively little about genetic adaptation of phytoplankton to these environments. Compared to its coastal relative Thalassiosira pseudonana, the oceanic diatom Thalassiosira oceanica is highly tolerant to iron limitation. The adaptation to low-iron conditions in T. oceanica has been attributed to a decrease in the photosynthetic components that are rich in iron. Genomic information on T. oceanica may shed light on the genetic basis of the physiological differences between the two species. The complete 141790 bp sequence of the T. oceanica chloroplast genome [GenBank: GU323224], assembled from massively parallel pyrosequencing (454) shotgun reads, revealed that the petF gene encoding for ferredoxin, which is localized in the chloroplast genome in T. pseudonana and other diatoms, has been transferred to the nucleus in T. oceanica. The iron-sulfur protein ferredoxin, a key element of the chloroplast electron transport chain, can be replaced by the iron-free flavodoxin under iron-limited growth conditions thereby contributing to a reduction in the cellular iron requirements. From a comparison to the genomic context of the T. pseudonana petF gene, the T. oceanica ortholog can be traced back to its chloroplast origin. The coding potential of the T. oceanica chloroplast genome is comparable to that of T. pseudonana and Phaeodactylum tricornutum, though a novel expressed ORF appears in the genomic region that has been subjected to rearrangements linked to the petF gene transfer event. The transfer of the petF from the cp to the nuclear genome in T. oceanica represents a major difference between the two closely related species. The ability of T. oceanica to tolerate iron limitation suggests that the transfer of petF from the chloroplast to the nuclear genome might have contributed to the ecological success of this species.
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影响因子：
14.9
作者：
Benson DA;Karsch-Mizrachi I;Lipman DJ;Ostell J;Wheeler DL
通讯作者：
Wheeler DL
影响因子：
56.9
作者：
Armbrust, EV;Berges, JA;Rokhsar, DS
通讯作者：
Rokhsar, DS
影响因子：
1.1
作者：
Fujita, Kiyohito;Ehira, Shigeki;Ohta, Niji
通讯作者：
Ohta, Niji
影响因子：
2.8
作者：
GUILLARD, RR;RYTHER, JH
通讯作者：
RYTHER, JH
影响因子：
64.8
作者：
Huang, CY;Ayliffe, MA;Timmis, JN
通讯作者：
Timmis, JN