The signal sequence suffices to direct export of outer membrane protein OmpA of Escherichia coli K-12
The signal sequence suffices to direct export of outer membrane protein OmpA of Escherichia coli K-12
复制标题
该信号序列足以直接输出大肠杆菌K-12的外膜蛋白OmpA
作者：
R. Freudl;H. Schwarz;M. Degen;U. Henning
我们研究了出口所需的信息是否存在于大肠杆菌325-残基外膜蛋白OmpA的成熟形式中。我们先前分析了ompA基因中重叠的内部缺失，结果使我们得出结论，如果存在这样的信息，它必须重复存在于蛋白质的膜部分，包括氨基酸残基1至177（R. Freudl，H.施瓦茨，M.克洛泽，N。R. Movva和U. Henning，EMBO J. 4：3593-3598，1985）。构建去除OmpA蛋白质的氨基酸残基1至229的密码子的缺失。在该构建体中，信号序列与蛋白质的周质部分融合。将所得的蛋白质（命名为Pro-OmpA delta 1-229）进行加工，并且成熟的95个残基的蛋白质在周质中积累。因此，输出所需的信息不存在于OmpA蛋白中。
We studied whether information required for export is present within the mature form of the Escherichia coli 325-residue outer membrane protein OmpA. We had previously analyzed overlapping internal deletions in the ompA gene, and the results allowed us to conclude that if such information exists it must be present repeatedly within the membrane part of the protein encompassing amino acid residues 1 to 177 (R. Freudl, H. Schwarz, M. Klose, N. R. Movva, and U. Henning, EMBO J. 4:3593-3598, 1985). A deletion which removed the codons for amino acid residues 1 to 229 of the OmpA protein was constructed. In this construct the signal sequence was fused to the periplasmic part of the protein. The resulting protein, designated Pro-OmpA delta 1-229, was processed, and the mature 95-residue protein accumulated in the periplasm. Hence, information required for export does not exist within the OmpA protein.
OmpA 蛋白的基因结构，OmpA 蛋白是细胞与细胞相互作用所需的大肠杆菌的主要表面蛋白。
影响因子：
5.6
作者：
Movva,NR;Nakamura,K;Inouye,M
通讯作者：
Inouye,M