Quantification of Diethyl Phthalate by a Rapid and Homogenous Fluorescence Polarization Immunoassay
Quantification of Diethyl Phthalate by a Rapid and Homogenous Fluorescence Polarization Immunoassay
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通过快速均质荧光偏振免疫分析对邻苯二甲酸二乙酯进行定量
DOI：
10.1080/00032719.2015.1060600
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发表时间：
2015-08
影响因子：
2
通讯作者：
Xi, Rimo*
中科院分区：
文献类型：
--
作者：
Eremin, Sergei A.;Deng, Chuan;Yin, Yongmei;Xi, Rimo*
A rapid and homogenous fluorescence polarization immunoassay (FPIA) for diethyl phthalate is reported. Two haptens and three immunogens for diethyl phthalate were synthesized to generate polyclonal antibodies and fluorescein isothiocyanate labeled diethyl phthalate was prepared as the tracer. The antibodies, methanol concentration, and incubation time were optimized. The cross-reactivities of the optimized antibodies to other phthalate esters were characterized. The antibody shows excellent sensitivity with a limit of detection of 6.0 ng mL−1 and a half-inhibition concentration of diethyl phthalate of 40.4 ng mL−1. The immunoassay had a linear dynamic range from 10 to 200 ng mL−1. The mean coefficient of variation was lower than 11.3% for intra-assay and lower than 14.6% for inter-assay measurements. The limit of detection was 1.46 ng mL−1 in bottled drinking water, 0.34 µg mL−1 in juice, and 50.0 µg g−1 in pharmaceutical capsules. The recovery was from 85.9 to 114.9%, and the coefficients of variation were 4.3 to 17.0%. The developed immunoassay provided good sensitivity, specificity, accuracy, and may serve as an alternative for diethyl phthalate determination for field screening of food, pharmaceuticals, and environmental materials.
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影响因子：
6.2
作者：
Hui Zhang;Xiaoqing Chen;Xin-yu Jiang
通讯作者：
Hui Zhang;Xiaoqing Chen;Xin-yu Jiang
影响因子：
6.1
作者：
Zhanhui Wang;Suxia Zhang;I. S. Nesterenko;S. Eremin;Jianzhong Shen
通讯作者：
Zhanhui Wang;Suxia Zhang;I. S. Nesterenko;S. Eremin;Jianzhong Shen
影响因子：
4.3
作者：
Russo, Mario Vincenzo;Notardonato, Ivan;Avino, Pasquale
通讯作者：
Avino, Pasquale
一种简单、快速、高通量的荧光偏振免疫分析方法，可同时检测蔬菜和环境水样中的有机磷农药
影响因子：
6.2
作者：
Xu, Zhen-Lin;Wang, Qiang;Sun, Yuan-Ming
通讯作者：
Sun, Yuan-Ming
影响因子：
2
作者：
Lee, Maw-Rong;Lai, Feng-Ying;Chung, Li-Wen
通讯作者：
Chung, Li-Wen