Kinetics of immune responses to SARS-CoV-2 proteins in individuals with varying severity of infection and following a single dose of the AZD1222.
Kinetics of immune responses to SARS-CoV-2 proteins in individuals with varying severity of infection and following a single dose of the AZD1222.
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DOI：
10.1093/cei/uxac009
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发表时间：
2022-06-23
影响因子：
4.6
通讯作者：
Malavige GN
中科院分区：
文献类型：
--
作者：
Jayathilaka D;Jeewandara C;Gomes L;Jayadas TTP;Kamaladasa A;Somathilake G;Guruge D;Pushpakumara PD;Ranasinghe T;Aberathna IS;Danasekara S;Gunathilaka B;Kuruppu H;Wijewickrama A;Wijayamuni R;Schimanski L;Tan TK;Ogg GS;Townsend A;Malavige GN
为了表征不同 SARS-CoV-2 蛋白的 IgG 和 IgA 反应，我们研究了斯里兰卡个体在自然感染后和单剂量 AZD1222 (Covishield) 后对 SARS-CoV-2 的抗体反应。在发病后或疫苗接种后 4 周和 12 周时，评估患者对 S1、S2、RBD 和 N 蛋白的 IgG 和 IgA 反应。在这些队列中还评估了 SARS-CoV-2 野生型 (WT)、α、β 和 λ 受体结合域的抗体以及 ACE2（血管紧张素转换酶 2）受体阻断抗体。对于轻症患者和疫苗接种者来说，对 S1、S2、RBD 和 N 蛋白的 IgG 反应从 4 周增加到 12 周，而中度/重度疾病患者的 IgG 反应保持不变。在疫苗中，S2 亚基的 IgG 抗体的显着上升幅度最高 (P < 0.0001)。与自然感染的疫苗相比，疫苗对所有测试的 SARS-CoV-2 蛋白的 IgA 抗体要低几倍。第 12 周时，疫苗中的血凝试验 (HAT) 滴度显着低于 α，轻症患者和疫苗中的血凝试验 (HAT) 滴度显着低于 β 和 λ。在中度/重度疾病患者中没有发现这种差异。疫苗对 SARS-CoV-2 的 IgA 显着减少，但 IgG 反应与自然感染的疫苗相当。然而，单剂疫苗后，针对挥发性有机化合物的抗体水平降低，并随着时间的推移进一步下降，这表明在因挥发性有机化合物而爆发疫情的国家，有必要缩小两剂疫苗之间的差距。轻度感染者、疫苗接种者 (AZD1222) 和未暴露者的抗体水平与 S2 相似。轻度感染者对 S2 的抗体反应最高，而中度/重度感染者对受体结合域的反应最高。与自然感染者相比，疫苗接种者对所有测试的 SARS-CoV-2 蛋白的 IgA 抗体要低几倍。
To characterize the IgG and IgA responses to different SARS-CoV-2 proteins, we investigated the antibody responses to SARS-CoV-2 following natural infection and following a single dose of AZD1222 (Covishield), in Sri Lankan individuals. The IgG and IgA responses were assessed to S1, S2, RBD, and N proteins in patients at 4 weeks and 12 weeks since the onset of illness or following vaccination. Antibodies to the receptor-binding domain of SARS-CoV-2 wild type (WT), α, β, and λ and ACE2 (Angiotensin Converting Enzyme 2) receptor blocking antibodies were also assessed in these cohorts. For those with mild illness and in vaccines, the IgG responses to S1, S2, RBD, and N protein increased from 4 weeks to 12 weeks, while it remained unchanged in those with moderate/severe illness. In the vaccines, IgG antibodies to the S2 subunit had the highest significant rise (P < 0.0001). Vaccines had several-fold lower IgA antibodies to all the SARS-CoV-2 proteins tested than those with natural infection. At 12 weeks, the haemagglutination test (HAT) titres were significantly lower to the α in vaccines and significantly lower in those with mild illness and in vaccines to β and for λ. No such difference was seen in those with moderate/severe illness. Vaccines had significantly less IgA to SARS-CoV-2, but comparable IgG responses those with natural infection. However, following a single dose vaccines had reduced antibody levels to the VOCs, which further declined with time, suggesting the need to reduce the gap between the two doses, in countries experiencing outbreaks due to VOCs. Those with mild infection, vaccinees (AZD1222) and unexposed individuals had similar antibody levels to S2. Those with mild infection had highest antibody responses to S2, while hose with moderate/severe illness had highest responses to the receptor binding domain. Vaccinees had several fold lower IgA antibodies to all the SARS-CoV-2 proteins tested than those with natural infection.
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DOI：
10.1016/s0140-6736(21)00183-5
发表时间：
2021-02-06
期刊：
Lancet (London, England)
影响因子：
--
作者：
Sabino EC;Buss LF;Carvalho MPS;Prete CA Jr;Crispim MAE;Fraiji NA;Pereira RHM;Parag KV;da Silva Peixoto P;Kraemer MUG;Oikawa MK;Salomon T;Cucunuba ZM;Castro MC;de Souza Santos AA;Nascimento VH;Pereira HS;Ferguson NM;Pybus OG;Kucharski A;Busch MP;Dye C;Faria NR
通讯作者：
Faria NR
IGA主导对SARS-COV-2的早期中和抗体反应。
影响因子：
17.1
作者：
Sterlin D;Mathian A;Miyara M;Mohr A;Anna F;Claër L;Quentric P;Fadlallah J;Devilliers H;Ghillani P;Gunn C;Hockett R;Mudumba S;Guihot A;Luyt CE;Mayaux J;Beurton A;Fourati S;Bruel T;Schwartz O;Lacorte JM;Yssel H;Parizot C;Dorgham K;Charneau P;Amoura Z;Gorochov G
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Gorochov G
DOI：
10.15557/pimr.2020.0003
发表时间：
2020-01-01
影响因子：
3.6
作者：
通讯作者：
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DOI：
10.1016/j.ijid.2021.06.031
发表时间：
2021-08
期刊：
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
影响因子：
--
作者：
Kamaladasa A;Gunasekara B;Jeewandara C;Jayathilaka D;Wijewickrama A;Guruge D;Wijayamuni R;Tan TK;Ogg GS;Townsend A;Malavige GN
通讯作者：
Malavige GN
影响因子：
16.6
作者：
Townsend A;Rijal P;Xiao J;Tan TK;Huang KA;Schimanski L;Huo J;Gupta N;Rahikainen R;Matthews PC;Crook D;Hoosdally S;Dunachie S;Barnes E;Street T;Conlon CP;Frater J;Arancibia-Cárcamo CV;Rudkin J;Stoesser N;Karpe F;Neville M;Ploeg R;Oliveira M;Roberts DJ;Lamikanra AA;Tsang HP;Bown A;Vipond R;Mentzer AJ;Knight JC;Kwok AJ;Screaton GR;Mongkolsapaya J;Dejnirattisai W;Supasa P;Klenerman P;Dold C;Baillie JK;Moore SC;Openshaw PJM;Semple MG;Turtle LCW;Ainsworth M;Allcock A;Beer S;Bibi S;Skelly D;Stafford L;Jeffrey K;O'Donnell D;Clutterbuck E;Espinosa A;Mendoza M;Georgiou D;Lockett T;Martinez J;Perez E;Gallardo Sanchez V;Scozzafava G;Sobrinodiaz A;Thraves H;Joly E
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Joly E