Heterogeneity of paclitaxel distribution in different tumor models assessed by MALDI mass spectrometry imaging.
Heterogeneity of paclitaxel distribution in different tumor models assessed by MALDI mass spectrometry imaging.
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DOI：
10.1038/srep39284
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发表时间：
2016-12-21
影响因子：
4.6
通讯作者：
Morosi L
中科院分区：
文献类型：
--
作者：
Giordano S;Zucchetti M;Decio A;Cesca M;Fuso Nerini I;Maiezza M;Ferrari M;Licandro SA;Frapolli R;Giavazzi R;Maurizio D;Davoli E;Morosi L
抗癌药物在实体瘤中的渗透对于保证治疗效果至关重要，因此需要了解药物在肿瘤不同部位分布的方法。质谱成像（MSI）具有良好的空间分辨率和上级特异性，在器官和肿瘤组织中显示药物分布方面具有很大的潜力。我们提出了一种准确和可重复的成像方法来研究药物在实体瘤不同部位的分布变化。应用该方法研究紫杉醇在具有不同组织病理学特征的三种卵巢癌模型以及结肠癌（HCT 116）、乳腺癌（MDA-MB-231）和恶性胸膜间皮瘤（MPM 487）中的分布。从MALDI成像结果和图像分析中可以明显看出肿瘤中的异质性药物渗透。各种模型之间的差异并不总是与经典HPLC分析检测的肿瘤匀浆中药物含量的显著变化有关。该方法的特异性也阐明了从定量角度分析的药物分布的异质性，突出了实体瘤不同部分中紫杉醇量的差异有多显著。
The penetration of anticancer drugs in solid tumors is important to ensure the therapeutic effect, so methods are needed to understand drug distribution in different parts of the tumor. Mass spectrometry imaging (MSI) has great potential in this field to visualize drug distribution in organs and tumor tissues with good spatial resolution and superior specificity. We present an accurate and reproducible imaging method to investigate the variation of drug distribution in different parts of solid tumors. The method was applied to study the distribution of paclitaxel in three ovarian cancer models with different histopathological characteristics and in colon cancer (HCT116), breast cancer (MDA-MB-231) and malignant pleural mesothelioma (MPM487). The heterogeneous drug penetration in the tumors is evident from the MALDI imaging results and from the images analysis. The differences between the various models do not always relate to significant changes in drug content in tumor homogenate examined by classical HPLC analysis. The specificity of the method clarifies the heterogeneity of the drug distribution that is analyzed from a quantitative point of view too, highlighting how marked are the variations of paclitaxel amounts in different part of solid tumors.
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影响因子：
4.3
作者：
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通讯作者：
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影响因子：
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作者：
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