Allelic diversity and phylogeny of homB, a novel co-virulence marker of Helicobacter pylori.
Allelic diversity and phylogeny of homB, a novel co-virulence marker of Helicobacter pylori.
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DOI：
10.1186/1471-2180-9-248
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发表时间：
2009-12-02
期刊：
影响因子：
4.2
通讯作者：
Monteiro L
中科院分区：
文献类型：
--
作者：
Oleastro M;Cordeiro R;Ménard A;Yamaoka Y;Queiroz D;Mégraud F;Monteiro L
homB 基因是幽门螺杆菌疾病标记候选者，与消化性溃疡病密切相关，而其旁系同源基因 homA 具有 90% 的序列同一性，与非溃疡性消化不良相关。 HomB 编码的外膜蛋白被证明有助于幽门螺杆菌的促炎特性，并且还参与细菌粘附。本研究调查了东亚和西方国家455株幽门螺杆菌中homB和homA基因的分布，并进行了序列比较和系统发育分析。 homB 和 homA 基因在世界范围内分布不均匀，东亚和西方菌株之间存在显着差异。对 homB 和 homA 序列的分析揭示了拷贝数及其基因组定位的多样性，东亚和西方菌株呈现不同的基因型。此外，homB 和 homA 序列分析表明通过相位变化进行调节。它还表明可能的重组事件，导致基因复制或 homB/homA 转换，这也可能与这些基因的调节有关。 homB 和 homA 的系统发育重建揭示了根据菌株地理起源的聚类。尽管任何等位基因与疾病之间没有相关性，但 homB 和 homA 均观察到基因中部区域的等位基因多样性。对于每个基因，检测到了一个占主导地位的全球等位基因，这表明 homB/homA 等位基因变异与菌株的地理起源无关。此外，所有等位基因均被证明在体内表达。总体而言，这些结果表明 homB 和 homA 基因是幽门螺杆菌 OMP 库的良好候选者，与宿主-细菌界面有关，并且还有助于抗原变异的产生，从而参与幽门螺杆菌的持久性。
The homB gene is a Helicobacter pylori disease-marker candidate, strongly associated with peptic ulcer disease, while homA, its paralogue gene with 90% sequence identity, is correlated with non-ulcer dyspepsia. The HomB encoded outer membrane protein was shown to contribute to the proinflammatory properties of H. pylori and also to be involved in bacterial adherence. This study investigated the distribution of homB and homA genes in 455 H. pylori strains from East Asian and Western countries, and carried out sequence comparison and phylogenetic analyses. Both homB and homA genes were heterogeneously distributed worldwide, with a marked difference between East Asian and Western strains. Analysis of homB and homA sequences revealed diversity regarding the number of copies and their genomic localization, with East Asian and Western strains presenting different genotypes. Moreover, homB and homA sequence analysis suggests regulation by phase variation. It also indicates possible recombination events, leading to gene duplication or homB/homA conversion which may as well be implicated in the regulation of these genes. Phylogenetic reconstruction of homB and homA revealed clustering according to the geographic origin of strains. Allelic diversity in the middle region of the genes was observed for both homB and homA, although there was no correlation between any allele and disease. For each gene, a dominant worldwide allele was detected, suggesting that homB/homA allelic variants were independent of the geographical origin of the strain. Moreover, all alleles were demonstrated to be expressed in vivo. Overall, these results suggest that homB and homA genes are good candidates to be part of the pool of H. pylori OMPs implicated in host-bacteria interface and also contributing to the generation of antigenic variability, and thus involved in H. pylori persistence.
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影响因子：
4.2
作者：
Oleastro M;Cordeiro R;Yamaoka Y;Queiroz D;Mégraud F;Monteiro L;Ménard A
通讯作者：
Ménard A
影响因子：
24.5
作者：
Maeda, S;Ogura, K;Omata, M
通讯作者：
Omata, M
影响因子：
24.5
作者：
Parsonnet, J;Friedman, GD;Vogelman, H
通讯作者：
Vogelman, H
影响因子：
2.6
作者：
Lehours, Philippe;Dupouy, Sandrine;Menard, Armelle
通讯作者：
Menard, Armelle
影响因子：
5
作者：
Nomura, AMY;Pérez-Pérez, GI;Blaser, MJ
通讯作者：
Blaser, MJ