Characterization of a novel class A carbapenemase PAD-1 from Paramesorhizobium desertii A-3-E(T), a strain highly resistant to β-lactam antibiotics.
Characterization of a novel class A carbapenemase PAD-1 from Paramesorhizobium desertii A-3-E(T), a strain highly resistant to β-lactam antibiotics.
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从paramemohizobium dedestii a-3-e（t）的新型A类A级Carbapenemase Pad的表征，这是一种对β-内酰胺抗生素高度抗性的菌株。
DOI：
10.1038/s41598-017-07841-1
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发表时间：
2017-08-21
影响因子：
4.6
通讯作者：
Song Y
中科院分区：
文献类型：
--
作者：
Lv R;Guo J;Yan Y;Chen R;Xiao L;Wang M;Fang N;Fang C;Cui Y;Yang R;Song Y
尽管临床抗生素耐药细菌引起了微生物学界的极大关注，但自然环境中持续存在的耐药细菌却长期被忽视。我们之前提出了一种从中国塔克拉玛干沙漠土壤中分离出来的新种Paramesorhizobium Desertii，它对大多数β-内酰胺类抗生素具有高度耐药性。为了鉴定该细菌中潜在的 β-内酰胺酶，我们首先使用改良的 Hodge 测定法确认了 P. Desertii A-3-ET 培养物冻融上清液中的碳青霉烯酶活性。然后，我们通过对上清液和基因组信息进行鸟枪式蛋白质组学分析，在菌株 A-3-ET 中鉴定出一种新型染色体编码的碳青霉烯酶 (PAD-1)。生物信息学分析表明PAD-1是A类碳青霉烯酶。随后用纯化的 PAD-1 进行的酶动力学测定证实了其碳青霉烯酶活性，该活性与具有临床意义的 A 类碳青霉烯酶（包括 BKC-1 和 KPC-2）的活性相似。由于 A-3-ET 的分离位置不受人类活动的影响，因此 PAD-1 不太可能与现代抗生素施加的选择压力相关。这项研究证实了环境抗性组中抗生素抗性决定因素的多样性。
Although clinical antibiotic-resistant bacteria have attracted tremendous attention in the microbiology community, the resistant bacteria that persist in natural environments have been overlooked for a longtime. We previously proposed a new species Paramesorhizobium desertii, isolated from the soil of the Taklimakan Desert in China that is highly resistant to most β-lactam antibiotics. To identify potential β-lactamase(s) in this bacteria, we first confirmed the carbapenemase activity in the freeze–thawed supernatant of a P. desertii A-3-ET culture using the modified Hodge assay. We then identified a novel chromosome-encoded carbapenemase (PAD-1) in strain A-3-ET, using a shotgun proteomic analysis of the supernatant and genomic information. The bioinformatics analysis indicated that PAD-1 is a class A carbapenemase. Subsequent enzyme kinetic assays with purified PAD-1 confirmed its carbapenemase activity, which is similar to that of clinically significant class A carbapenemases, including BKC-1 and KPC-2. Because the location in which A-3-ET was isolated is not affected by human activity, PAD-1 is unlikely to be associated with the selection pressures exerted by modern antibiotics. This study confirmed the diversity of antibiotic-resistant determinants in the environmental resistome.
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影响因子：
5.2
作者：
Hocquet, Didier;Colomb, Melanie;Meziane-Cherif, Djalal
通讯作者：
Meziane-Cherif, Djalal
影响因子：
4.9
作者：
JORIS, B;LEDENT, P;FRERE, JM
通讯作者：
FRERE, JM
影响因子：
2.9
作者：
Ke, Wei;Bethel, Christopher R.;van den Akker, Focco
通讯作者：
van den Akker, Focco
影响因子：
4.9
作者：
Nicoletti, Adriana Giannini;Marcondes, Marcelo F. M.;Gales, Ana Cristina
通讯作者：
Gales, Ana Cristina
影响因子：
14.2
作者：
Paterson, DL
通讯作者：
Paterson, DL