A TATA binding protein regulatory network that governs transcription complex assembly.
A TATA binding protein regulatory network that governs transcription complex assembly.
复制标题
控制转录复合物组件的TATA结合蛋白调节网络。
DOI：
10.1186/gb-2007-8-4-r46
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发表时间：
2007
期刊：
影响因子：
12.3
通讯作者：
Pugh, B. Franklin
中科院分区：
文献类型：
--
作者：
Huisinga, Kathryn L.;Pugh, B. Franklin
一部分的组装过程中涉及整个酵母基因组的TATA结合蛋白（TBP）的调节建模和实验测试。真核基因由蛋白质控制，蛋白质逐步组装成转录复合物。生物化学定义的各个组装步骤如何协调并应用于整个基因组在很大程度上是未知的。在这里，我们的模型和实验测试的一部分，涉及整个酵母基因组的TATA结合蛋白（TBP）的调节装配过程。生物化学知识被用来制定一系列耦合TBP调控反应，涉及TFIID，佐贺，NC 2，Mot1，和启动子DNA。然后将这些反应与转录周期的基本部分联系起来，并进行计算建模。采用单一框架，允许特定步骤的贡献因基因而异。使用ChIP芯片和表达微阵列测定在全基因组范围内测量启动子结合和转录输出。突变被用来通过关闭网络的特定部分来测试框架。该模型占大多数转录活性启动子TBP的调节，并提供了一个概念性的工具，用于解释全基因组数据集。这些发现进一步证明了TBP调控在全基因组范围内的相互联系。
A portion of the assembly process involving the regulation of the TATA binding protein (TBP) throughout the yeast genome is modeled and experimentally tested. Eukaryotic genes are controlled by proteins that assemble stepwise into a transcription complex. How the individual biochemically defined assembly steps are coordinated and applied throughout a genome is largely unknown. Here, we model and experimentally test a portion of the assembly process involving the regulation of the TATA binding protein (TBP) throughout the yeast genome. Biochemical knowledge was used to formulate a series of coupled TBP regulatory reactions involving TFIID, SAGA, NC2, Mot1, and promoter DNA. The reactions were then linked to basic segments of the transcription cycle and modeled computationally. A single framework was employed, allowing the contribution of specific steps to vary from gene to gene. Promoter binding and transcriptional output were measured genome-wide using ChIP-chip and expression microarray assays. Mutagenesis was used to test the framework by shutting down specific parts of the network. The model accounts for the regulation of TBP at most transcriptionally active promoters and provides a conceptual tool for interpreting genome-wide data sets. The findings further demonstrate the interconnections of TBP regulation on a genome-wide scale.
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影响因子：
10.5
作者：
AUBLE, DT;HANSEN, KE;HAHN, S
通讯作者：
HAHN, S
影响因子：
5.3
作者：
Bai, Y;Perez, GM;Weil, PA
通讯作者：
Weil, PA
DOI：
10.1073/pnas.202236699
发表时间：
2002-10-01
影响因子：
11.1
作者：
Cang, Y;Prelich, G
通讯作者：
Prelich, G
影响因子：
16
作者：
Coleman, RA;Taggart, AKP;Pugh, BF
通讯作者：
Pugh, BF
DOI：
10.1073/pnas.052397899
发表时间：
2002-03-05
影响因子：
11.1
作者：
Dasgupta, A;Darst, RP;Auble, DT
通讯作者：
Auble, DT