Visualization of bacteriophage P1 infection by cryo-electron tomography of tiny Escherichia coli.
Visualization of bacteriophage P1 infection by cryo-electron tomography of tiny Escherichia coli.
复制标题
DOI：
10.1016/j.virol.2011.06.005
复制
发表时间：
2011-09-01
期刊：
影响因子：
3.7
通讯作者：
Margolin W
中科院分区：
文献类型：
--
作者：
Liu J;Chen CY;Shiomi D;Niki H;Margolin W
噬菌体P1有一条可收缩的尾巴，针对宿主外膜表面保守的脂多糖进行初始吸附。P1DNA进入宿主细胞的机制还不是很清楚，主要是因为噬菌体和细菌之间的瞬时分子相互作用很难用常规方法来研究。在这里，我们设计了微小的大肠杆菌宿主细胞，以便通过冷冻电子断层扫描可以前所未有的细节捕捉到P1-宿主相互作用的初始阶段。对冷冻水化标本的三维重建分析表明，有三种主要构型：在噬菌体头部有DNA的延长尾部阶段，有DNA的收缩尾部阶段，以及没有DNA的收缩尾部阶段。对不同构象的比较分析表明，在尾部收缩过程中，内尾管均匀地穿透到大肠杆菌周质中，基板明显远离外膜。
Bacteriophage P1 has a contractile tail that targets the conserved lipopolysaccharide on the outer membrane surface of the host for initial adsorption. The mechanism by which P1 DNA enters the host cell is not well understood, mainly because the transient molecular interactions between bacteriophage and bacteria have been difficult to study by conventional approaches. Here, we engineered tiny E. coli host cells so that the initial stages of P1-host interactions could be captured in unprecedented detail by cryo-electron tomography. Analysis of three-dimensional reconstructions of frozen-hydrated specimens revealed three predominant configurations: an extended tail stage with DNA present in the phage head, a contracted tail stage with DNA, and a contracted tail stage without DNA. Comparative analysis of various conformations indicated that there is uniform penetration of the inner tail tube into the E. coli periplasm and a significant movement of the baseplate away from the outer membrane during tail contraction.
登录
查看更多内容
影响因子：
64.8
作者：
Liu, Jun;Bartesaghi, Alberto;Borgnia, Mario J.;Sapiro, Guillermo;Subramaniam, Sriram
通讯作者：
Subramaniam, Sriram
DOI：
10.1073/pnas.0400444101
发表时间：
2004-04-20
影响因子：
11.1
作者：
Fokine, A;Chipman, PR;Rossmann, MG
通讯作者：
Rossmann, MG
影响因子：
3.4
作者：
CASPAR, DLD
通讯作者：
CASPAR, DLD
影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
影响因子：
3.2
作者：
FURUKAWA, H;KUROIWA, T;MIZUSHIMA, S
通讯作者：
MIZUSHIMA, S