Lipase Activity in the Larval Midgut of Rhynchophorus palmarum: Biochemical Characterization and the Effects of Reducing Agents.
Lipase Activity in the Larval Midgut of Rhynchophorus palmarum: Biochemical Characterization and the Effects of Reducing Agents.
复制标题
DOI：
10.3390/insects8030100
复制
发表时间：
2017-09-13
期刊：
影响因子：
3
通讯作者：
Grillo LAM
中科院分区：
文献类型：
--
作者：
Santana CC;Barbosa LA;Júnior IDB;Nascimento TGD;Dornelas CB;Grillo LAM
脂肪酶在昆虫脂质获取、储存和动员中起着关键作用，也是昆虫许多生理过程的基础。脂质是昆虫饮食的重要组成部分，它们在中肠腔中被水解、吸收，并用于合成复杂的脂质。南美棕榈象甲（Rhynchophorus palmarum）是商业棕榈种植园的重要害虫之一。然而，关于这种昆虫的脂质消化的研究很少。在这项工作中，我们描述了R. palmarum棕榈象后中肠脂肪酶活性的生化表征。脂肪酶活性在温度为37℃~ 45℃和pH为6.5时最高。脂肪酶活性对盐和钙浓度的变化也很敏感。脂肪酶在结构上被描述为具有Ser-His-Asp催化三元组的酶，含有一个活性丝氨酸。丝氨酸蛋白酶抑制剂PMSF （phenylmethane sulfonyl fluoride）抑制了棕榈褐霉脂肪酶的活性，证明了丝氨酸残基对这种活性的重要性。脂肪酶水解不同链长的对硝基苯酯的能力揭示了广泛底物的活性。在还原性谷胱甘肽（GSH）和二硫苏糖醇（DTT）的作用下，棕榈黄酮的脂肪酶活性升高，而在氧化性谷胱甘肽（GSSG）的作用下，脂肪酶活性急剧降低。据我们所知，本研究首次提供了棕榈象中脂肪酶活性的信息。
Lipases have key roles in insect lipid acquisition, storage, and mobilization and are also fundamental to many physiological processes in insects. Lipids are an important component of insect diets, where they are hydrolyzed in the midgut lumen, absorbed, and used for the synthesis of complex lipids. The South American palm weevil Rhynchophorus palmarum is one of the most important pests on commercial palm plantations. However, there are few studies about lipid digestion for this insect. In this work, we have described the biochemical characterization of the lipase activity in the posterior midgut of the R. palmarum palm weevil. Lipase activity was highest between the temperatures of 37 °C and 45 °C and at pH 6.5. Lipase activity was also sensitive to variations in salt and calcium concentrations. Lipases have been described structurally as enzymes with the Ser-His-Asp Catalytic Triad, containing an active serine. The serine protease inhibitor PMSF (phenylmethane sulfonyl fluoride) inhibited the lipases from R. palmarum, demonstrating the importance of a serine residue for this activity. The ability of the lipases to hydrolyze p-Nitrophenyl esters with different chain lengths has revealed the activities of a broad range of substrates. The lipase activities of R. palmarum increased in the presence of reduced glutathione (GSH) and dithiothreitol (DTT), while in the presence of oxidized glutathione (GSSG), activities were drastically reduced. To our knowledge, this study has provided the first information about lipase activity in the R. palmarum palm weevil.
登录
查看更多内容
影响因子：
4.1
作者：
SMITH, GM;ROTHWELL, K;BOWNES, M
通讯作者：
BOWNES, M
影响因子：
3.8
作者：
Auerswald, L;Siegert, KJ;Gäde, G
通讯作者：
Gäde, G
DOI：
10.1016/0020-1790(87)90147-8
发表时间：
1987-01-01
期刊：
INSECT BIOCHEMISTRY
影响因子：
--
作者：
HIRAYAMA, Y;CHINO, H
通讯作者：
CHINO, H
DOI：
10.1016/0005-2760(88)90333-5
发表时间：
1988-11-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
JURENKA, RA;STANLEYSAMUELSON, DW;BLOMQUIST, GJ
通讯作者：
BLOMQUIST, GJ
影响因子：
5.4
作者：
Ponnuvel, KM;Nakazawa, H;Yamakawa, M
通讯作者：
Yamakawa, M