Expression of OPN3 in acral lentiginous melanoma and its associated with clinicohistopathologic features and prognosis.
Expression of OPN3 in acral lentiginous melanoma and its associated with clinicohistopathologic features and prognosis.
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OPN3在肢端雀斑样痣黑色素瘤中的表达及其与临床病理特征和预后的关系
DOI：
10.1002/iid3.438
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发表时间：
2021-09
期刊：
影响因子：
--
通讯作者：
Lu H
中科院分区：
文献类型：
--
作者：
Zeng W;Zhang W;Feng J;He X;Lu H
最近在两种肺癌亚型中描述了与转移相关的OPN3上调。在表皮黑素细胞中识别出的光不依赖性功能的OPN3已经显示出了希望。然而，在恶性黑素细胞组织中，OPN3的表达和表征仍然是未知的。我们研究了与肢端雀斑样黑色素瘤（ALM）OPN3表达相关的临床组织病理学特征，这是一种罕见的皮肤黑色素瘤亚型，与既往日光暴露无关。共84例交界性黑色素细胞痣（JMN，n = 12）、原发性ALMs（n = 39）和来自ALMs的腹股沟淋巴结转移（ILNM，n = 23）的样本评价了OPN3的免疫组织化学表达。使用定量真实的时间PCR、多重免疫荧光和蛋白质印迹分析进一步确定黑素细胞肿瘤中的OPN3信使RNA和蛋白质水平。我们还评估了OPN3表达与临床病理特征和预后之间的关系。通过免疫组织化学分析，与JMN和ALM相反，ILNM具有更高的OPN3表达评分（p < .001）。高OPN3评分与溃疡的存在、Breslow深度和Clark水平增加相关（分别为p = 0.025、p = 0.042和p = 0.012）。此外，当比较等于或大于100和小于100组之间的免疫组化评分的OPN3表达时，发现患者总生存率的显著差异（p = 0.037）。此外，考克斯回归模型显示，高OPN3评分与黑色素瘤生存率较差相关。OPN3高表达与ALMs和转移表型以及不良预后显著相关。肢端雀斑样痣性黑色素瘤由于诊断延迟、侵犯性生物学行为而预后不良。然而，其与预后相关的分子特征在很大程度上仍然未知。我们已经检测到OPN3在ALMs中的表达特征，并证明高OPN3是一个阴性预后指标。
OPN3 upregulation associated with metastasis was recently described in two subtypes of lung cancers. And OPN3 identified in light‐independent functions in epidermal melanocytes has already shown promise. However, in malignant melanocytic tissues, the expression and characterization of OPN3 remain uncharacterized. We investigated the clinico‐histopathologic features in relation to OPN3 expression of acral lentiginous melanoma (ALM), which is a rare cutaneous melanoma subtype and not associated with prior sunlight exposure. In all, 84 samples of junctional melanocytic nevi (JMN, n = 12), primary ALMs (n = 39) and inguinal lymph node metastasis (ILNM, n = 23) from ALMs were evaluated for the immunohistochemical expression of OPN3. OPN3 messenger RNA and protein level were further determined in melanocytic tumors using quantitative real‐time PCR, multiimmunofluorescence and Western blot assays. We also estimated the associations OPN3 expression between clinicopathological features and prognosis. ILNMs, in contrast to JMN and ALMs, had higher OPN3 expression scores (p < .001) by immunohistochemistry analysis. High OPN3 score was associated with presence of ulceration, increased Breslow depth and Clark level (p = .025, p = .042, and p = .012, respectively). Furthermore, a remarkable difference (p = .037) of patient overall survival was found when comparing the OPN3 expression of immunohistochemical score between equal to or larger than 100 and below 100 groups. Also, Cox regression models showed that high OPN3 scores were associated with worse melanoma survival. High OPN3 expression is significantly associated with ALMs and metastatic phenotype as well as a poor prognosis. Acral lentiginous melanoma has a poor prognosis due to delayed diagnosis aggressive biological behavior. However, its molecule features associated with prognosis remains largely unknown. We have detected the expression characteristic of OPN3 in ALMs, and demonstrated that high OPN3 is a negative prognostic indicator.
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