Amperometric Electrochemical Microsystem for a Miniaturized Protein Biosensor Array
Amperometric Electrochemical Microsystem for a Miniaturized Protein Biosensor Array
复制标题
用于小型化蛋白质生物传感器阵列的电流电化学微系统
DOI：
--
复制
发表时间：
2009
影响因子：
5.1
通讯作者：
A. Mason
中科院分区：
文献类型：
--
作者：
Chao Yang;Yue Huang;B. Hassler;R. Mark Worden;A. Mason
基于蛋白质的生物电化学接口为快速检测、连续使用和小型化传感器阵列提供了巨大的潜力。本文介绍了一个微系统平台，使多个生物电化学接口，同时询问芯片上的安培读出系统。描述了一种后互补金属氧化物半导体（CMOS）制造过程，其允许在电极上形成平面电极阵列和生物传感器界面的自组装。片上，0.5妈妈CMOS读出电子包括一个紧凑的稳压器，支持非常广泛的输入电流范围-6 pA至10 μ A-以适应不同的生物传感器接口。该2.3倍2.2毫米芯片采用5 V电源供电，电流为0.6 mA。采用循环伏安法对电化学传感器原型平台进行了表征。氧化峰值与溶液中目标分析物浓度之间的线性关系验证了系统的功能性，并展示了该平台在高灵敏度、低成本和基于蛋白质的芯片传感器阵列中的未来实现潜力。
Protein-based bioelectrochemical interfaces offer great potential for rapid detection, continuous use, and miniaturized sensor arrays. This paper introduces a microsystem platform that enables multiple bioelectrochemical interfaces to be interrogated simultaneously by an onchip amperometric readout system. A post-complementary metal-oxide semiconductor (CMOS) fabrication procedure is described that permits the formation of planar electrode arrays and self assembly of biosensor interfaces on the electrodes. The onchip, 0.5-mum CMOS readout electronics include a compact potentiostat that supports a very broad range of input currents-6 pA to 10 muA-to accommodate diverse biosensor interfaces. The 2.3 times 2.2-mm chip operates from a 5-V supply at 0.6 mA. A prototype electrochemical sensor platform, including an onchip potentiostat and miniaturized biosensor array, was characterized by using cyclic voltammetry. The linear relationship between the oxidization peak values and the concentrations of target analytes in the solution verifies functionality of the system and demonstrates the potential for future implementations of this platform in high sensitivity, low cost, and onchip protein-based sensor arrays.
DOI：
10.1073/pnas.93.20.10614
发表时间：
1996-10-01
影响因子：
11.1
作者：
Schena, M;Shalon, D;Davis, RW
通讯作者：
Davis, RW