Tracer coupling patterns of the ganglion cell subtypes in the mouse retina.
Tracer coupling patterns of the ganglion cell subtypes in the mouse retina.
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DOI：
10.1002/cne.21912
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发表时间：
2009-02-10
期刊：
影响因子：
--
通讯作者：
Bloomfield SA
中科院分区：
文献类型：
--
作者：
Völgyi B;Chheda S;Bloomfield SA
现在很清楚，通过缝隙连接的电耦合在整个视网膜上普遍存在，由五种主要神经元类型中的每一种表达。随着选择性缝隙连接蛋白缺失突变体的出现，小鼠已成为研究视网膜神经元间耦联功能的重要模型。在这项研究中，我们通过注射差距连接永久性示踪剂Neurobiotin来检测神经节细胞的示踪剂偶联模式，首次提供了野生型小鼠视网膜神经节细胞偶联的全面调查。小鼠神经节细胞根据体细胞参数分为22个形态学上不同的亚型。大多数（16/22）神经节细胞亚型与邻近的神经节和/或无长突细胞示踪剂偶联。与神经节细胞偶联的无长突细胞呈多轴或宽视野形态，具有广泛的乔木。我们发现，不同亚型的神经节细胞从来没有相互耦合，这表明它们具有独立的电网络。最后，我们发现，22个神经节细胞群体的示踪剂耦合模式在71个视网膜研究中基本上是刻板的。我们的研究结果表明，电耦合在小鼠视网膜内层广泛存在，这表明缝隙连接在视觉信息处理中起着重要作用。
It is now clear that electrical coupling via gap junctions isprevalent across the retina, expressed by each of the fivemain neuronal types. With the introduction of mutants inwhich selective gap junction connexins are deleted, themouse has recently become an important model for studyingthe function of coupling between retinal neurons. In thisstudy we examined the tracer-coupling pattern of ganglioncells by injecting them with the gap junction-permanenttracer Neurobiotin to provide, for the first time, a comprehensivesurvey of ganglion cell coupling in the wildtypemouse retina. Murine ganglion cells were differentiated into22 morphologically distinct subtypes based on somadendriticparameters. Most (16/22) ganglion cell subtypes were tracer-coupled to neighboring ganglion and/or amacrinecells. The amacrine cells coupled to ganglion cellsdisplayed either polyaxonal or wide-field morphologies withextensive arbors. We found that different subtypes of ganglioncells were never coupled to one another, indicatingthat they subserved independent electrical networks. Finally, we found that the tracer-coupling patterns of the 22ganglion cell populations were largely stereotypic acrossthe 71 retinas studied. Our results indicate that electricalcoupling is extensive in the inner retina of the mouse, suggestingthat gap junctions play essential roles in visual information processing.
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