Roles of sulfur compounds in growth and alkaline phosphatase activities of Microcystis aeruginosa under phosphorus deficiency stress
Roles of sulfur compounds in growth and alkaline phosphatase activities of Microcystis aeruginosa under phosphorus deficiency stress
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缺磷胁迫下硫化合物对铜绿微囊藻生长和碱性磷酸酶活性的影响
DOI：
10.1007/s11356-020-08480-2
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发表时间：
2020-04
影响因子：
5.8
通讯作者：
Dayi Zhang
中科院分区：
文献类型：
--
作者：
En Xie;Fangfang Li;Chaozi Wang;Shi Wei;Chen Huang;Keyu Fa;Xiao Zhao;Dayi Zhang
铜绿微囊藻是富营养化水体中最常见的藻类之一。在缺磷胁迫下，铜绿微囊藻可以产生碱性磷酸酶（AKP）来利用有机磷，因此AKP可以作为藻类生长的重要指标。含硫化合物在水体中普遍存在，但对含硫化合物与铜绿微囊藻相互作用的研究有限。本研究引入33种含硫化合物培养铜绿微囊藻，结果表明藻类生长与AKP活性呈正相关。在定量构效关系预测的基础上，利用毒性评价软件工具进一步评价有机硫化合物的毒性。藻类生长结果与毒性终点有很强的相关性，表明有机硫化合物作为有毒物质抑制藻类生长。根据每个样品的藻类生长和AKP活性的结果，随后通过Python进行K-means聚类分析，统计上，含硫化合物可以充分聚为2类。从聚类结果来看，磺酸的毒性较低，而硫氨基酸的毒性较强。研究了33种不同类型的含硫化合物与常见藻类铜绿微囊藻的相互作用。K-means聚类分析和相关分析表明，藻类生长和碱性磷酸酶活性与预测的毒性终点有很强的相关性。
Microcystis aeruginosa is one of the most common algae found in eutrophicated water bodies. Alkaline phosphatase (AKP) can be produced by Microcystis aeruginosa to utilize organic phosphates under phosphorus deficiency stress, thereby AKP can be regarded as an important indicator for algal growth. Sulfur compounds are ubiquitous in waters, while investigation on the interactions between sulfur compounds and Microcystis aeruginosa is limited. In this work, we introduced 33 types of sulfur compounds to culture Microcystis aeruginosa, and the results demonstrated that algal growth is positively related to AKP activities. Toxicity of organic sulfur compounds was further evaluated using Toxicity Estimation Software Tool based on quantitative structure-activity relationship prediction. The algal growth results exhibited strong correlation to the toxicity endpoints suggesting the organic sulfur compounds inhibits the algal growth as toxic matters. K-means cluster analyses have been carried out subsequently via Python based on the results of algal growth and AKP activities of each sample and statistically, the sulfur compounds can be adequately clustered into 2 groups. According to clustering results, sulfonic acids exhibit low toxicity while sulfur amino acids can be considered as more toxic compounds. Graphical abstract Varied sulfur compounds (33 types) were investigated to find out the interactions between them and Microcystis aeruginosa, a common alga. K-means cluster and correlation analyses demonstrate that algal growth and alkaline phosphatase activities exhibited strong correlation to the predicted toxicity endpoints.
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