Quantification of trivalent non-replicating rotavirus vaccine antigens in the presence of aluminum adjuvant.
Quantification of trivalent non-replicating rotavirus vaccine antigens in the presence of aluminum adjuvant.
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DOI：
10.1016/j.jim.2021.113056
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发表时间：
2021-07
影响因子：
2.2
通讯作者：
White JA
中科院分区：
文献类型：
--
作者：
McAdams D;Lakatos K;Estrada M;Chen D;Plikaytis B;Sitrin R;White JA
Parenterally administered rotavirus vaccines may overcome the low efficacy observed in resource-poor regions that use live oral formulations. We have reported work on a trivalent nonreplicating rotavirus vaccine (NRRV) for parenteral administration consisting of the recombinant tetanus toxoid P2 CD4 epitope fused to a truncated VP8* fragment (P2-VP8*) for the P[4], P[6], and P[8] serotypes of rotavirus adjuvanted with aluminum. An essential part of developing this vaccine candidate was devising quantification methods for each antigen in the trivalent NRRV in the presence of aluminum adjuvant. This report describes the development of quantitative inhibition enzyme-linked immunosorbent assays (ELISAs) for in vitro antigenicity determination of the adjuvanted trivalent NRRV using serotype-specific monoclonal antibodies (mAbs) against each of the P2-VP8* antigens. Adjuvanted trivalent vaccine samples are titrated and incubated with a constant concentration of specific mAbs against each NRRV P2-VP8* antigen variant. Unbound mAbs are measured by ELISA to indirectly quantify the amount of each antigen present in the trivalent vaccine. Sensitive, specific, and reproducible inhibition ELISAs were developed and qualified for each antigen and used for final product quantification and release testing without desorption of the vaccine antigen.
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人轮状病毒重组 VP8* 亚基肠外疫苗候选物的构建和表征。
影响因子：
5.5
作者：
Wen, Xiaobo;Cao, Dianjun;Jones, Ronald W.;Li, Jianping;Szu, Shousun;Hoshino, Yasutaka
通讯作者：
Hoshino, Yasutaka
影响因子：
3.1
作者：
Church JA;Parker EP;Kosek MN;Kang G;Grassly NC;Kelly P;Prendergast AJ
通讯作者：
Prendergast AJ
影响因子：
11.8
作者：
Tate, Jacqueline E.;Burton, Anthony H.;Parashar, Umesh D.
通讯作者：
Parashar, Umesh D.
DOI：
10.1016/s1473-3099(17)30242-6
发表时间：
2017-08
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
Groome MJ;Koen A;Fix A;Page N;Jose L;Madhi SA;McNeal M;Dally L;Cho I;Power M;Flores J;Cryz S
通讯作者：
Cryz S
影响因子：
5.5
作者：
Kirkwood CD;Ma LF;Carey ME;Steele AD
通讯作者：
Steele AD