Cell-cell adhesion and morphogenesis in Dictyostelium discoideum.
Cell-cell adhesion and morphogenesis in Dictyostelium discoideum.
复制标题
盘基网柄菌的细胞间粘附和形态发生。
DOI：
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
R. Kamboj
中科院分区：
文献类型：
--
作者：
C. Siu;R. Kamboj
在盘基网柄藻的发育过程中，细胞在聚集阶段获得EDTA抗性的细胞-细胞粘附。EDTA抗性细胞结合活性与Mr 80，000（gp 80）的细胞表面糖蛋白相关，其通过嗜同性相互作用介导细胞-细胞结合。从cDNA序列推导的gp 80的结构的分析揭示了在NH 2-末端结构域中存在三个内部同源片段，其也包含与神经细胞粘附分子同源的区域。二级结构预测显示大量的β-结构和非常少的α-螺旋。这通过圆二色性测量证实。很可能同源片段被组织成球状结构，通过富含Pro的茎结构域从细胞表面延伸。gp 80的细胞结合活性位于NH 2末端结构域的第一个球状重复序列内，并已定位于Val 123和Leu 173之间的51个氨基酸区域。已经制备了与该区域内的序列相对应的合成寡肽，并测定了它们与细胞表面gp 80结合的能力。结果导致该区域内的一个八肽序列的嗜同性结合位点的鉴定。含有该八肽序列的合成肽和针对该位点的单价抗体在聚集期间阻断有组织的细胞流的形成。虽然细胞聚集体最终形成，但大多数未能经历进一步的发展，产生蛞蝓和子实体，表明涉及gp 80的细胞-细胞粘附是正常形态发生中的重要步骤。
During development of Dictyostelium discoideum, cells acquire EDTA-resistant cell-cell adhesion at the aggregation stage. The EDTA-resistant cell binding activity is associated with a cell surface glycoprotein of Mr 80,000 (gp80), which mediates cell-cell binding via homophilic interaction. Analysis of the structure of gp80 deduced from cDNA sequence reveals the presence of three internally homologous segments in the NH2-terminal domain, which also contains regions with homology to the neural cell adhesion molecule. Secondary structure predictions show an abundance of beta-structures and very few alpha-helices. This is confirmed by circular dichroism measurements. It is likely that the homologous segments are organized into globular structures, extended from the cell surface by a Pro-rich stalk domain. The cell binding activity of gp80 resides within the first globular repeat of the NH2-terminal domain and has been mapped to a 51 amino acid region between Val123 and Leu173. Synthetic oligopeptides corresponding to sequences within this region have been prepared and assayed for their ability to bind to cell surface gp80. Results lead to identification of the homophilic binding site to an octapeptide sequence within this region. Synthetic peptides containing this octapeptide sequence and univalent antibodies directed against this site block the formation of organized cell streams during aggregation. Although cell aggregates are eventually formed, most fail to undergo further development to give rise to slugs and fruiting bodies, indicating that cell-cell adhesion involving gp80 is an important step in normal morphogenesis.
影响因子：
56.9
作者：
CUNNINGHAM, BA;HEMPERLY, JJ;EDELMAN, GM
通讯作者：
EDELMAN, GM
DOI：
10.1042/bj2440001
发表时间：
1987-05
期刊：
The Biochemical journal
影响因子：
--
作者：
Martin G. Low
通讯作者：
Martin G. Low
DOI：
10.1073/pnas.83.24.9822
发表时间：
1986-12-01
影响因子：
11.1
作者：
HEMPERLY, JJ;EDELMAN, GM;CUNNINGHAM, BA
通讯作者：
CUNNINGHAM, BA