The Effect of EGR1 on the Proliferation of Dermal Papilla Cells.
The Effect of EGR1 on the Proliferation of Dermal Papilla Cells.
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EGR1对真皮乳头细胞增殖的影响
DOI：
10.3390/genes13071242
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发表时间：
2022-07-14
期刊：
影响因子：
3.5
通讯作者：
Sun W
中科院分区：
文献类型：
--
作者：
Xu Y;Wang S;Cao X;Yuan Z;Getachew T;Mwacharo JM;Haile A;Lv X;Sun W
早期生长反应因子1 （EGR1）是一种锌指转录因子，在毛囊发育中起着至关重要的作用。根据我们之前的研究，EGR1是骨形态发生蛋白7 （bone morphogenetic protein 7, BMP7）的转录启动子，BMP7是参与真皮乳头细胞增殖的候选基因。毛囊是小羊皮图案形成的基础，真皮乳头细胞（dermal papilla cells, DPCs）对毛囊的生长起着重要作用，为了阐明EGR1在毛囊和毛囊中的作用，本研究旨在探讨EGR1在DPCs中的生物学作用。本研究首先通过聚合酶链反应克隆了EGR1编码序列（CDS）区域，并进行了生物信息学分析。然后，采用5-乙基-2′-脱氧尿苷（EDU）和细胞计数试剂盒-8 （CCK8）检测EGR1的功能，并采用Western blot （WB）分析EGR1对DPCs的细胞作用。通过qPCR和WB检测其在增殖标志基因PCNA和CDK2上的表达，进一步证实了EGR1对DPCs的增殖作用。成功克隆了一只羔羊的EGR1 CDS区序列，并对其核酸序列进行了分析，发现其与褐家鼠、小家鼠、牛牛和智人高度同源。对EGR1编码蛋白的预测分析表明，它是一种膜外蛋白，而不是分泌蛋白，在细胞核和细胞质中具有亚细胞定位。EGR1上调DPCs的增殖作用显著强于对照组（p < 0.01），而EGR1下调DPCs的增殖作用则相反。与对照组相比，增殖标志物包括PCNA和CDK2在EGR1基因过表达中也出现了差异上调，而在EGR1基因下调中则出现了相反的结果。综上所述，我们的研究揭示了EGR1促进DPCs的增殖，我们推测EGR1可能与毛囊生长发育密切相关。
Early growth response factor 1 (EGR1) is a zinc-finger transcription factor that plays a vital role in the development of hair follicles. According to our previous studies, EGR1 is a transcriptional promoter of the bone morphogenetic protein 7 (BMP7), a candidate gene involved in the proliferation of dermal papilla cells. Since hair follicles are the basis of lambskin pattern formation and dermal papilla cells (DPCs) act on hair follicle growth, in order to elucidate the role of EGR1 and hair follicles, this study aimed to investigate the biological role of EGR1 in DPCs. In our study, the EGR1 coding sequence (CDS) region was firstly cloned by polymerase chain reaction, and bioinformatics analysis was performed. Then, the function of EGR1 was detected by 5-ethynyl-2’-deoxyuridine (EDU) and Cell Counting Kit-8 (CCK8), and Western blot (WB) was conducted to analyze the cellular effect of EGR1 on DPCs. The proliferative effect of EGR1 on DPCs was also further confirmed by detecting its expression by qPCR and WB on marker genes of proliferation, including PCNA and CDK2. The sequence of the EGR1 CDS region of a lamb was successfully cloned, and its nucleic acid sequence was analyzed and found to be highly homologous to Rattus norvegicus, Mus musculus, Bos taurus and Homo sapiens. Predictive analysis of the protein encoded by EGR1 revealed that it is an extra-membrane protein, and not a secretory protein, with subcellular localization in the nucleus and cytoplasm. The proliferative effect of DPCs was significantly stronger (p < 0.01) in EGR1 up-regulated DPCs compared to the controls, while the opposite result was observed in EGR1 down-regulated DPCs. Markers of proliferation including PCNA and CDK2 also appeared to be differentially upregulated in EGR1 gene overexpression compared to the controls, with the opposite result in EGR1 gene downregulation. In summary, our study revealed that EGR1 promotes the proliferation of DPCs, and we speculate that EGR1 may be closely associated with hair follicle growth and development.
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