Effects of caudal elevation on testicular function in rats. Separation of effects on spermatogenesis and steroidogenesis.
Effects of caudal elevation on testicular function in rats. Separation of effects on spermatogenesis and steroidogenesis.
复制标题
尾部抬高对大鼠睾丸功能的影响。
作者：
D. Deaver;R. P. Amann;R. Hammerstedt;R. Ball;D. Veeramachaneni;X. J. Musacchia
当暴露于微重力（空间飞行）超过7天时，各种生物过程都会受到干扰，包括睾丸功能。将大鼠悬挂在一种特殊的挽具中（尾部抬高），以引起胸腔内的血液汇集，并消除后肢的支撑功能，这是用来在地球上模拟空间飞行期间遇到的微重力效应。通常，这会诱导雄性大鼠隐睾。本研究采用三个实验来区分尾侧抬高（30度角）和睾丸解剖位置对精子发生和类固醇生成的影响。使用尾套（实验1和2）或全身套（实验3）将大鼠尾抬高7天。当使用尾带时，大鼠的睾丸落入腹腔，但腹股沟管的结扎阻止了这种重新定位。腹侧睾丸组大鼠睾丸重量减轻（P <0.05），睾丸组织学异常，尾吊组每克实质中精子细胞数低于对照组（P <0.05）。与此相反，精子发生不受尾部抬高在大多数大鼠腹股沟管结扎或在大鼠提高全身马具。无论采用何种方法抬高睾丸或睾丸解剖位置，悬吊大鼠血清和睾丸间质液中睾酮浓度均较低（P <0.05）。腹腔内睾丸组大鼠血清促黄体生成素浓度升高（P <0.05）。（250字处删节）
A variety of biologic processes are perturbed when exposed to microgravity (space flight) for more than 7 days, including testicular function. Suspension of rats in a special harness (caudal elevation) to induce thoracic pooling of blood fluids and remove the support function of the hind limbs is used to mimic, on earth, the effects of microgravity encountered during space flight. Typically, this induces cryptorchidism in male rats. Three experiments were conducted to differentiate the effects of caudal elevation (30 degrees angle) and anatomic location of testes on spermatogenesis and steroidogenesis. Rats were subjected to caudal elevation for 7 days using either a tail harness (experiments 1 and 2) or a whole-body harness (experiment 3). Testes of rats fell into the abdominal cavity when a tail harness was used, but ligation of the inguinal canal prevented this repositioning. For rats with abdominal testes, testicular weight was reduced (P less than 0.05) and histology of testes was abnormal; the number of spermatids per gram parenchyma was lower (P less than 0.05) in tail-suspended rats compared with control rats. In contrast, spermatogenesis was not affected by caudal elevation in most rats in which the inguinal canal was ligated or in rats elevated by whole-body harness. Concentrations of testosterone in serum and testicular interstitial fluid were lower (P less than 0.05) in suspended rats, regardless of the method used for caudal elevation or anatomic location of testes. Concentrations of luteinizing hormone in serum were elevated (P less than 0.05) in rats with intra-abdominal testes.(ABSTRACT TRUNCATED AT 250 WORDS)
影响因子：
4.8
作者：
ZIRKIN, BR;SANTULLI, R;EWING, LL
通讯作者：
EWING, LL
合成代谢类固醇对超负荷和超负荷悬浮骨骼肌的影响。
DOI：
10.1152/jappl.1987.63.5.2128
发表时间：
1987
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Tsika,RW;Herrick,RE;Baldwin,KM
通讯作者：
Baldwin,KM