Highly transmissible cytoplasmic incompatibility by the extracellular insect symbiont Spiroplasma.
Highly transmissible cytoplasmic incompatibility by the extracellular insect symbiont Spiroplasma.
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DOI：
10.1016/j.isci.2022.104335
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发表时间：
2022-05-20
期刊：
影响因子：
5.8
通讯作者：
Gottlieb, Yuval
中科院分区：
文献类型：
--
作者：
Pollmann, Marie;Moore, Logan D.;Krimmer, Elena;D'Alvise, Paul;Hasselmann, Martin;Perlman, Steve J.;Ballinger, Matthew J.;Steidle, Johannes L. M.;Gottlieb, Yuval
细胞质不相容性（CI）是一种由母体遗传的内共生体感染节肢动物（如沃尔巴克氏体）引起的生殖操纵形式，感染的雄性和未感染的雌性之间的交配产生很少或没有后代。我们报告发现了一个新的CI共生体，一株螺旋体引起CI的寄生蜂Lariophagus dendus。它的细胞外发生使我们能够建立CI在未感染的成年昆虫通过转移螺原体感染的血淋巴。我们对CI-螺原体基因组进行了测序，没有发现任何在沃尔巴克氏体中发现的导致CI的cif基因的同源物，这表明CI是独立进化的。相反，基因组包含其他潜在的导致CI的候选基因，例如在真核生物发育中至关重要但在细菌基因组中罕见的高迁移率族（HMG）盒蛋白的同源物。螺原体的细胞外性质和广泛的宿主范围，包括医学和农业上重要的节肢动物，使其成为一个有前途的工具，研究CI及其应用。一株螺旋体菌株在寄生蜂中诱导细胞质不相容性（CI）螺旋体和CI通过血淋巴注射有效地转移到幼稚宿主中CI-螺旋体基因组含有潜在的CI引起基因，但没有Wolbachia cifs螺旋体基因组中cif基因的缺失表明CI的独立起源动物学;昆虫学;微生物学;微生物学寄生虫
Cytoplasmic incompatibility (CI) is a form of reproductive manipulation caused by maternally inherited endosymbionts infecting arthropods, like Wolbachia, whereby matings between infected males and uninfected females produce few or no offspring. We report the discovery of a new CI symbiont, a strain of Spiroplasma causing CI in the parasitoid wasp Lariophagus distinguendus. Its extracellular occurrence enabled us to establish CI in uninfected adult insects by transferring Spiroplasma-infected hemolymph. We sequenced the CI-Spiroplasma genome and did not find any homologues of any of the cif genes discovered to cause CI in Wolbachia, suggesting independent evolution of CI. Instead, the genome contains other potential CI-causing candidate genes, such as homologues of high-mobility group (HMG) box proteins that are crucial in eukaryotic development but rare in bacterial genomes. Spiroplasma’s extracellular nature and broad host range encompassing medically and agriculturally important arthropods make it a promising tool to study CI and its applications. A Spiroplasma strain induces cytoplasmic incompatibility (CI) in a parasitoid wasp Spiroplasma and CI are efficiently transferred to naive hosts by hemolymph injection The CI-Spiroplasma genome contains potential CI-causing genes, but no Wolbachia cifs Absence of cif genes in the Spiroplasma genome suggests an independent origin of CI Zoology; Entomology; Microbiology; Microbiology parasite
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