Effect of rhBMP-2 and VEGF in a vascularized bone allotransplant experimental model based on surgical neoangiogenesis.
Effect of rhBMP-2 and VEGF in a vascularized bone allotransplant experimental model based on surgical neoangiogenesis.
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DOI：
10.1002/jor.22277
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发表时间：
2013-04
期刊：
影响因子：
--
通讯作者：
Bishop AT
中科院分区：
文献类型：
--
作者：
Mattar T;Friedrich PF;Bishop AT
我们已经证明活体异体骨的存活无需长期免疫抑制，使用短期免疫抑制和同时创建一个自体新生循环。在这项研究中，骨形态发生蛋白-2 （rhBMP-2）和/或血管内皮生长因子（VEGF）被用来增强这一过程。将46只黑鼠股骨骨干骨异位移植至46只Lewis大鼠。同种异体移植营养蒂微血管修复结合自体隐静脉动静脉束（AV）髓内植入和含有缓冲液（对照）、rhBMP-2或rhBMP-2 + VEGF的可生物降解微球。每天给予FK-506，连续14天维持血管生成过程中营养蒂的流动。在18周的生存期后，我们测量了房室束的血管生成（毛细血管密度）和皮质骨血流。联合治疗组（rhBMP-2 + VEGF）两项指标均高于对照组（p<0.05）。rhBMP-2 + VEGF组成骨细胞计数明显高于对照组（p<0.05）。与对照组相比，rhBMP2 + VGF组和rhBMP2组都有更大的骨形成趋势（p=0.059）。在带血管的同种异体骨移植物中，局部给予VEGF和rhBMP-2增强了供体来源的植入血管的血管生成、成骨细胞活性和骨血流量。
We have demonstrated survival of living allogeneic bone without long-term immunosuppression using short-term immunosuppression and simultaneous creation of an autogenous neoagiogenic circulation. In this study bone morphogenic protein-2 (rhBMP-2), and/or vascular endothelial growth factor (VEGF), were used to augment this process. Femoral diaphyseal bone was transplanted heterotopically from 46 Dark Agouti to 46 Lewis rats. Microvascular repair of the allotransplant nutrient pedicle was combined with intra-medullary implantation of an autogenous saphenous arteriovenous (AV) bundle and biodegradable microspheres containing buffer (control), rhBMP-2 or rhBMP-2 + VEGF. FK-506 given daily for 14 days maintained nutrient pedicle flow during angiogenesis. After an 18 weeks survival period, we measured angiogenesis (capillary density) from the AV bundle and cortical bone blood flow. Both measures were greater in the combined (rhBMP-2 + VEGF) group than rhBMP-2 and control groups (p<0.05). Osteoblast counts were also higher in the rhBMP-2 + VEGF group (p<0.05). A trend towards greater bone formation was seen in both rhBMP2 + VGF and rhBMP2 groups as compared to controls (p=0.059). Local administration of VEGF and rhBMP-2 augments angiogenesis, osteoblastic activity and bone blood flow from implanted blood vessels of donor origin in vascularized bone allografts.
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影响因子：
4.1
作者：
Patel, Zarana S.;Young, Simon;Tabata, Yasuhiko;Jansen, John A.;Wong, Mark E. K.;Mikos, Antonios G.
通讯作者：
Mikos, Antonios G.
DOI：
10.1016/0006-291x(92)92277-5
发表时间：
1992-12-15
影响因子：
3.1
作者：
PEPPER, MS;FERRARA, N;MONTESANO, R
通讯作者：
MONTESANO, R
影响因子：
2.8
作者：
Pelzer, Michael;Larsen, Mikko;Bishop, Allen T.
通讯作者：
Bishop, Allen T.
影响因子：
2.8
作者：
Larsen, Mikko;Pelzer, Michael;Bishop, Allen T.
通讯作者：
Bishop, Allen T.
影响因子：
4.9
作者：
Kempen, DHR;Lu, LC;Yaszemski, MJ
通讯作者：
Yaszemski, MJ