Investigation of the Potential Mechanisms Underlying Nuclear F-Actin Organization in Ovarian Cancer Cells by High-Throughput Screening in Combination With Deep Learning.
Investigation of the Potential Mechanisms Underlying Nuclear F-Actin Organization in Ovarian Cancer Cells by High-Throughput Screening in Combination With Deep Learning.
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DOI：
10.3389/fcell.2022.869531
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发表时间：
2022
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
作者：
越来越多的证据支持丝状肌动蛋白（F-actin）和球状肌动蛋白存在于体细胞核中的观点，并参与染色质重塑、基因转录调控和DNA损伤修复。然而，核F-肌动蛋白如何在细胞中聚合的潜在机制仍然不完全清楚。在这里，我们使用小分子抑制剂库筛选结合深度学习方法，确定了参与卵巢癌细胞核F-肌动蛋白聚合的潜在激酶靶点。对本研究中使用的抑制剂的靶点的分析表明，PI 3 K-AKT通路参与调节卵巢癌细胞中的核F-肌动蛋白组织。我们的工作为揭示核F-肌动蛋白在卵巢癌中的重要作用以及了解核F-肌动蛋白结构是如何组织的奠定了基础。
Increasing evidence supports the notion that filamentous actin (F-actin) and globular actin exist in the nuclei of somatic cells, and are involved in chromatin remodeling, gene transcription regulation and DNA damage repair. However, the underlying mechanisms of how nuclear F-actin are polymerized in cells remain incompletely understood. Here, we identify potential kinase targets that participate in nuclear F-actin polymerization in ovarian cancer cells using small-molecule inhibitor library screening in combination with a deep learning approach. The analysis of the targets of the inhibitors used in this study suggest that the PI3K-AKT pathway are involved in regulating nuclear F-actin organization in ovarian cancer cells. Our work lays the foundation for uncovering the important roles of nuclear F-actin in the context of ovarian cancer, and for understanding how nuclear F-actin structures are organized.
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DOI：
10.1038/nrclinonc.2013.5
发表时间：
2013-04
期刊：
Nature reviews. Clinical oncology
影响因子：
--
作者：
通讯作者：
--
影响因子：
8.8
作者：
Fiore APZP;Spencer VA;Mori H;Carvalho HF;Bissell MJ;Bruni-Cardoso A
通讯作者：
Bruni-Cardoso A
影响因子：
56.9
作者：
Baarlink, Christian;Wang, Haicui;Grosse, Robert
通讯作者：
Grosse, Robert
影响因子：
48
作者：
Riedl, Julia;Crevenna, Alvaro H.;Wedlich-Soldner, Roland
通讯作者：
Wedlich-Soldner, Roland
影响因子：
10.3
作者：
du Bois, A;Lück, HJ;Pfisterer, J
通讯作者：
Pfisterer, J