Gain-of-function of the 1-aminocyclopropane-1-carboxylate synthase gene ACS1G induces female flower development in cucumber gynoecy.
Gain-of-function of the 1-aminocyclopropane-1-carboxylate synthase gene ACS1G induces female flower development in cucumber gynoecy.
复制标题
1-氨基环丙烷-1-羧酸合酶基因ACS1G的功能获得诱导黄瓜雌花期雌花发育
DOI：
10.1093/plcell/koaa018
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发表时间：
2021-04-17
期刊：
影响因子：
--
通讯作者：
Sun J
中科院分区：
文献类型：
--
作者：
Zhang H;Li S;Yang L;Cai G;Chen H;Gao D;Lin T;Cui Q;Wang D;Li Z;Cai R;Bai S;Lucas WJ;Huang S;Zhang Z;Sun J
摘要单性花为研究植物性别决定提供了一个有用的系统。在黄瓜(Cucumis Sativus L.)中，控制单性花发育的三个主要孟德尔基因座是雌性(F)、雄株[a；1-氨基环丙烷-1-羧酸(ACC)合成酶11，acs11]和单株(M；ACS2)，这里指的是雌雄同株(FAM)模型，结合两个基因，雌雄异株(g，WIP家族C2H2锌指转录因子基因Wip1)和乙烯生物合成基因ACC Oxin 2(ACO2)。F基因座是一个30.2kb的串联重复序列，包含3个基因，决定着雌核发育和提高果实产量的潜力。然而，决定女性表型的基因及其机制仍不清楚。在这里，我们创建了一组突变体，并揭示了ACS1G负责F基因座的雌核发育。这种复制导致ACS1G获得了一种新的启动子和表达模式；在携带F基因座重复的植物中，ACS1G在花芽发育早期表达，在那里它与ACO2一起作用产生乙烯爆发。由此产生的乙烯抑制Wip1并激活ACS2启动雌核发育。这种早期的ACS1G表达绕过了ACS11产生乙烯的需要，从而为雌性花的发育建立了一条优势途径。基于这些发现，我们提出了一个关于这些乙烯生物合成基因如何协同控制黄瓜单性花发育的模型。
Abstract Unisexual flowers provide a useful system for studying plant sex determination. In cucumber (Cucumis sativus L.), three major Mendelian loci control unisexual flower development, Female (F), androecious [a; 1-aminocyclopropane-1-carboxylate {ACC} synthase 11, acs11], and Monoecious (M; ACS2), referred to here as the Female, Androecious, Monoecious (FAM) model, in combination with two genes, gynoecious (g, the WIP family C2H2 zinc finger transcription factor gene WIP1) and the ethylene biosynthetic gene ACC oxidase 2 (ACO2). The F locus, conferring gynoecy and the potential for increasing fruit yield, is defined by a 30.2-kb tandem duplication containing three genes. However, the gene that determines the Female phenotype, and its mechanism, remains unknown. Here, we created a set of mutants and revealed that ACS1G is responsible for gynoecy conferred by the F locus. The duplication resulted in ACS1G acquiring a new promoter and expression pattern; in plants carrying the F locus duplication, ACS1G is expressed early in floral bud development, where it functions with ACO2 to generate an ethylene burst. The resulting ethylene represses WIP1 and activates ACS2 to initiate gynoecy. This early ACS1G expression bypasses the need for ACS11 to produce ethylene, thereby establishing a dominant pathway for female floral development. Based on these findings, we propose a model for how these ethylene biosynthesis genes cooperate to control unisexual flower development in cucumber.
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影响因子：
56.9
作者：
Boualem, Adnane;Fergany, Mohamed;Bendahmane, Abdelhafid
通讯作者：
Bendahmane, Abdelhafid
影响因子：
11.6
作者：
De Martinis, D;Mariani, C
通讯作者：
Mariani, C
DOI：
10.1073/pnas.76.1.170
发表时间：
1979-01-01
影响因子：
11.1
作者：
ADAMS, DO;YANG, SF
通讯作者：
YANG, SF
影响因子：
7.2
作者：
Garcia, Alicia;Aguado, Encarnacion;Jamilena, Manuel
通讯作者：
Jamilena, Manuel
影响因子：
7.4
作者：
IWAHORI, S;LYONS, JM;SMITH, OE
通讯作者：
SMITH, OE