Development of diet-induced insulin resistance in adult Drosophila melanogaster.
Development of diet-induced insulin resistance in adult Drosophila melanogaster.
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DOI：
10.1016/j.bbadis.2012.04.012
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发表时间：
2012-08
影响因子：
6.2
通讯作者：
Bauer, Johannes H.
中科院分区：
文献类型：
--
作者：
Morris, Siti Nur Sarah;Coogan, Claire;Chamseddin, Khalil;Fernandez-Kim, Sun Ok;Kolli, Santharam;Keller, Jeffrey N.;Bauer, Johannes H.
果蝇黑腹果蝇越来越多地被用作昂贵的啮齿动物模型的替代品来研究人类疾病。苍蝇模型适用于各种各样的人类疾病，如阿尔茨海默氏症和帕金森病，或心脏功能。该系统的优点是生成时间快，成本低。然而，苍蝇系统最大的优势是强大的遗传工具，可以快速解剖分子疾病机制。在这里，我们描述了成年果蝇代谢表型的饮食依赖性发展。根据营养物质的具体种类及其在饮食中的相对数量，果蝇表现出体重增加和储存大分子水平的变化。此外，在果蝇的主要代谢器官——脂肪体中，胰岛素信号的活性在过度摄食后会降低。当果蝇受到急性食物刺激时，过量进食的果蝇的胰岛素信号活性也会下降，这表明过量进食会导致胰岛素抵抗。在衰老的果蝇中也观察到类似的变化，胰岛素抵抗样表型的发展始于中年早期。综上所述，这些数据表明，不平衡的饮食破坏了成年黑腹海蝇的代谢稳态，并促进了胰岛素抵抗的表型。因此，果蝇系统可能是研究胰岛素抵抗分子机制和开发药物治疗方案的一个有用的替代工具。
The fruit fly Drosophila melanogaster is increasingly utilized as an alternative to costly rodent models to study human diseases. Fly models exist for a wide variety of human conditions, such as Alzheimer's and Parkinson’s Disease, or cardiac function. Advantages of the fly system are its rapid generation time and its low cost. However, the greatest strength of the fly system are the powerful genetic tools that allow for rapid dissection of molecular disease mechanisms. Here, we describe the diet-dependent development of metabolic phenotypes in adult fruit flies. Depending on the specific type of nutrient, as well as its relative quantity in the diet, flies show weight gain and changes in the levels of storage macromolecules. Furthermore, the activity of insulin-signaling in the major metabolic organ of the fly, the fat body, decreases upon overfeeding. This decrease in insulin-signaling activity in overfed flies is moreover observed when flies are challenged with an acute food stimulus, suggesting that overfeeding leads to insulin resistance. Similar changes were observed in aging flies, with the development of the insulin resistance-like phenotype beginning at early middle ages. Taken together, these data demonstrate that imbalanced diet disrupts metabolic homeostasis in adult D. melanogaster and promotes insulin-resistant phenotypes. Therefore, the fly system may be a useful alternative tool in the investigation of molecular mechanisms of insulin resistance and the development of pharmacologic treatment options.
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DOI：
10.18632/aging.100185
发表时间：
2010-08
期刊：
Aging
影响因子：
--
作者：
Haselton AT;Fridell YW
通讯作者：
Fridell YW
DOI：
10.1073/pnas.0807833105
发表时间：
2008-10-07
影响因子：
11.1
作者：
Kohyama-Koganeya, Ayako;Kim, Yeon-Jeong;Hirabayashi, Yoshio
通讯作者：
Hirabayashi, Yoshio
影响因子：
29
作者：
Birse RT;Choi J;Reardon K;Rodriguez J;Graham S;Diop S;Ocorr K;Bodmer R;Oldham S
通讯作者：
Oldham S
影响因子：
21.3
作者：
Lee, Kyu-Sun;Kwon, O-Yu;Yu, Kweon
通讯作者：
Yu, Kweon
DOI：
10.1073/pnas.0706121104
发表时间：
2007-08-14
影响因子：
11.1
作者：
Bauer, Johannes H.;Chang, Chengyi;Helfand, Stephen L.
通讯作者：
Helfand, Stephen L.