Doxycycline for transgene control disrupts gut microbiome diversity without compromising acute neuroinflammatory response.
Doxycycline for transgene control disrupts gut microbiome diversity without compromising acute neuroinflammatory response.
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DOI：
10.1186/s12974-023-03004-4
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发表时间：
2024-01-04
影响因子：
9.3
通讯作者：
中科院分区：
文献类型：
--
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The tetracycline transactivator (tTA) system provides controllable transgene expression through oral administration of the broad-spectrum antibiotic doxycycline. Antibiotic treatment for transgene control in mouse models of disease might have undesirable systemic effects resulting from changes in the gut microbiome. Here we assessed the impact of doxycycline on gut microbiome diversity in a tTA-controlled model of Alzheimer’s disease and then examined neuroimmune effects of these microbiome alterations following acute LPS challenge. We show that doxycycline decreased microbiome diversity in both transgenic and wild-type mice and that these changes persisted long after drug withdrawal. Despite the change in microbiome composition, doxycycline treatment had minimal effect on basal transcriptional signatures of inflammation the brain or on the neuroimmune response to LPS challenge. Our findings suggest that central neuroimmune responses may be less affected by doxycycline at doses needed for transgene control than by antibiotic cocktails at doses used for experimental microbiome disruption. The online version contains supplementary material available at 10.1186/s12974-023-03004-4.
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影响因子：
16.2
作者：
Hoover, Brian R.;Reed, Miranda N.;Su, Jianjun;Penrod, Rachel D.;Kotilinek, Linda A.;Grant, Marianne K.;Pitstick, Rose;Carlson, George A.;Lanier, Lorene M.;Yuan, Li-Lian;Ashe, Karen H.;Liao, Dezhi
通讯作者：
Liao, Dezhi
影响因子：
1.8
作者：
Boynton FDD;Ericsson AC;Uchihashi M;Dunbar ML;Wilkinson JE
通讯作者：
Wilkinson JE
DOI：
10.1016/j.ijantimicag.2017.03.017
发表时间：
2017-09-01
影响因子：
10.8
作者：
Becker, Eugenia;Schmidt, Thomas S. B.;Frey-Wagner, Isabelle
通讯作者：
Frey-Wagner, Isabelle
影响因子：
5.3
作者：
Born, Heather A.;Kim, Ji-Yoen;Jankowsky, Joanna L.
通讯作者：
Jankowsky, Joanna L.
影响因子：
15.8
作者：
Jankowsky JL;Slunt HH;Gonzales V;Savonenko AV;Wen JC;Jenkins NA;Copeland NG;Younkin LH;Lester HA;Younkin SG;Borchelt DR
通讯作者：
Borchelt DR