Clinicopathological and Immunomicroenvironment Characteristics of Epstein-Barr Virus-Associated Gastric Cancer in a Chinese Population.
Clinicopathological and Immunomicroenvironment Characteristics of Epstein-Barr Virus-Associated Gastric Cancer in a Chinese Population.
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中国人群EB病毒相关胃癌的临床病理和免疫微环境特征
DOI：
10.3389/fonc.2020.586752
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发表时间：
2020
影响因子：
4.7
通讯作者：
Ji J
中科院分区：
文献类型：
--
作者：
Jia X;Guo T;Li Z;Zhang M;Feng Y;Dong B;Li Z;Hu Y;Li Z;Xing X;Jia S;Ji J
EB病毒相关胃癌（EBVaGC）具有独特的肿瘤免疫微环境。我们对中国人群的 EBVaGC 队列中的肿瘤浸润免疫细胞进行了全面分析。对 1,328 例连续手术切除的胃癌病例进行了 Epstein-Barr 编码区 (EBER) 原位杂交。分别用组织微阵列中的 CD3、CD20、CD57 和 CD68 抗体进行免疫组织化学染色后，计算患者的免疫细胞（包括 T 细胞、B 细胞、自然杀伤细胞和巨噬细胞）的密度。 EBVaGC 患者占总人群的 4.1%（1,328 例中的 55 例）。 EBVaGC患者的平均年龄低于非EBVaGC患者。组织学上，EBVaGC患者表现出低分化腺癌（P = 0.004）和较低的血管侵犯频率（P = 0.034）。 EBVaGC 患者的 CD3+ T 淋巴细胞密度（CD3，23.84 ± 14.49 vs. 12.76 ± 8.93，P < 0.001）和 CD68+ 巨噬细胞（CD68，9.73 ± 5.25 vs. 5.44 ± 4.18，P < 0.001）密度显着较高。 CD3+ T 细胞密度可以更好地预测 EBVaGC 患者的 5 年总生存率 (P = 0.022)。 EBVaGC患者年龄较小，腺癌低分化，血管侵犯较少。多种免疫细胞浸润增加影响患者预后，尤其是CD3+T淋巴细胞较多的EBVaGC患者，生存时间更长。
Epstein–Barr virus-associated gastric cancer(EBVaGC)has a unique tumor immune microenvironment. We performed a comprehensive analysis of the tumor-infiltrating immune cells in a cohort of EBVaGC in a Chinese population. Epstein–Barr encoding region (EBER) in situ hybridization was performed in 1,328 consecutive cases of surgically resected GC. Densities of immune cells, including T cells, B cells, natural killer cells, and macrophages from the patients were calculated after immunohistochemical staining with CD3, CD20, CD57, and CD68 antibodies in tissue microarrays, respectively. EBVaGC patients accounted for 4.1% (55 of 1,328) cases in the overall population. The average age of patients with EBVaGC was lower than that of non-EBVaGC patients. Histologically, EBVaGC patients exhibited poorly differentiated adenocarcinoma (P = 0.004) and lower frequency of vascular invasion (P = 0.034). The density of CD3+ T lymphocytes (CD3, 23.84 ± 14.49 vs. 12.76 ± 8.93, P < 0.001) and CD68+ macrophages (CD68, 9.73 ± 5.25 vs. 5.44 ± 4.18, P < 0.001) was significantly higher in EBVaGC patients. CD3+ T cell density predicted better 5-year overall survival of EBVaGC patients (P = 0.022). EBVaGC patients were younger with low-differentiated adenocarcinoma and less vascular invasion. Increased infiltration of multiple immune cells affected the prognosis of patients, especially EBVaGC patients with more CD3+ T lymphocytes, who survived longer.
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影响因子：
2.5
作者：
Cho J;Kang MS;Kim KM
通讯作者：
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