Human immunodeficiency virus (HIV) nef-specific cytotoxic T lymphocytes in noninfected heterosexual contact of HIV-infected patients.
Human immunodeficiency virus (HIV) nef-specific cytotoxic T lymphocytes in noninfected heterosexual contact of HIV-infected patients.
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HIV感染者的非感染异性接触中的人类免疫缺陷病毒（HIV）nef特异性细胞毒性T淋巴细胞。
DOI：
10.1172/jci117085
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
É. Oksenhendler
中科院分区：
文献类型：
--
作者：
P. Langlade‐Demoyen;N. Ngo;F. Ferchal;É. Oksenhendler
我们报告检测HIV特异性细胞毒性T淋巴细胞（CTL）之间的23个经常合作伙伴的HIV感染者。46例受试者中15例HLA-A2.1分型阳性。在研究的23名接触者中，7人在重复检测中呈血清阳性，16人呈血清阴性。16名血清阴性接触者中无一人p24抗原血症阳性，淋巴细胞共培养试验也无阳性，但在两种情况下，可通过PCR检测到HIV-1 DNA（一种情况下使用gag SK 38/39引物，另一种情况下使用pol P3/P4引物）。这两个人保持血清阴性分别为18个月和36个月。在15名HLA-A2.1受试者（7名索引，2名血清阳性接触者和6名血清阴性接触者）和4名HLA匹配的HIV阴性供体中进行HIV特异性细胞毒性。在6个血清阴性接触者的外周血淋巴细胞未受刺激的大量培养物中，均未检测到env、gag或nef蛋白特异性CTL。然而，使用有限稀释测定，我们发现通常高频率的HIV env和gag的HIV nef特异性CTL前体（CTLp）与在血清阴性HLA匹配的健康供体中观察到的非常相似。因为在这些个体中没有HIV的存在，这些发现反对沉默HIV感染的可能性，并表明针对nef的CTL应答可能参与性暴露后病毒的快速有效清除。
We report on the detection of HIV-specific cytotoxic T lymphocytes (CTL) among 23 regular partners of HIV-infected individuals. 15 of the 46 individuals enrolled in the study were positive for HLA-A2.1 typing. Among the 23 contacts studied, 7 were seropositive and 16 were seronegative on repeated tests. None of the 16 seronegative contacts were positive for p24 antigenemia nor were they positive by the lymphocytes coculture assay, although, in two instances HIV-1 DNA could be detected by PCR (in one case using a gag SK 38/39 primer, and in the other using a primer for the pol P3/P4 primer). These two individuals remained seronegative for 18 and 36 mo, respectively. HIV-specific cytotoxicity was performed in the 15 HLA-A2.1 subjects (7 indexes, 2 seropositive contacts, and 6 seronegative contacts) and in 4 HLA-matched HIV negative donors. CTL specific for env, gag, or nef proteins could not be detected in unstimulated bulk cultures of peripheral blood lymphocytes in any of the six seronegative contacts. However, using a limiting dilution assay we found an usually high frequency of HIV nef-specific CTL precursors (CTLp) for HIV env and gag was very similar to that observed in seronegative HLA-matched healthy donors. Because no presence of HIV could be demonstrated in these individuals, these findings argue against the possibility of a silent HIV infection and suggest that a CTL response against nef may be involved in a rapid and effective clearance of the virus after sexual exposure.
新鲜淋巴细胞对提高 HIV-1 分离敏感性的影响：一项多中心研究。
DOI：
--
发表时间：
1990
期刊：
Journal of acquired immune deficiency syndromes
影响因子：
--
作者：
Farzadegan,H;Imagawa,D;Gupta,P;Lee,MH;Jacobson,L;Saah,A;Grovit,K;RinaldoJr,CR;Polk,BF
通讯作者：
Polk,BF
影响因子：
6.4
作者：
CLERICI, M;GIORGI, JV;SHEARER, GM
通讯作者：
SHEARER, GM
人类免疫缺陷病毒 1 型沉默感染：在高危异性恋人群中罕见发生。
影响因子：
20.3
作者：
Brettler,DB;Somasundaran,M;Forsberg,AF;Krause,E;Sullivan,JL
通讯作者：
Sullivan,JL