Drosophila Krüppel protein is a transcriptional represser
Drosophila Krüppel protein is a transcriptional represser
复制标题
果蝇 Krüppel 蛋白是一种转录抑制蛋白
作者：
J. Licht;M. Grossel;J. Figge;U. Hansen
KRÜPPEL（Kr）是一种合子活跃的果蝇分节基因，在胚胎发育的胚盘阶段在限制性结构域中表达，并参与控制果蝇胸节和腹节的发育1。Kr编码含有DNA结合锌指基序的多肽2，其破坏产生Kr突变体3，4。我们检测了野生型Kr蛋白以及Lac阻遏物/Kr融合蛋白在HeLa和CV-1细胞中的转录活性。野生型Kr和LacKr嵌合蛋白抑制来自报告启动子的转录，其中来自偶数跳跃启动子5内序列的共有Kr结合位点已插入上游位置。我们映射的阻遏功能的Kr的蛋白质的丙氨酸丰富的氨基末端区域，作为一个紫胶/Kr融合蛋白只包含氨基酸26110的Kr抑制转录从一个报告启动子含有上游紫胶运营商。这表明Kr蛋白的DNA结合和抑制活性是不同的。这些数据是一致的遗传证据，Kr抑制even-skipped 6，7和hunchback 8的表达，并建议，Kr是一个负调节果蝇的转录。
KRÜPPEL (Kr), one of the zygotically active Drosophila segmentation genes, is expressed in a restricted domain during the blastoderm stage of embryogenesis and is involved in the control of development of the thoracic and abdominal segments of the fly1. Kr encodes a polypeptide containing DNA-binding zinc-finger motifs2, disruptions of which yield Kr mutants3,4. We have assayed the transcriptional activities of wild-type Kr protein as well as Lac repressor/Kr fusion proteins in HeLa and CV-1 cells. Wild-type Kr and a LacKr chimaeric protein repressed transcription from reporter promoters in which a consensus Kr binding site derived from sequences within the even-skipped promoter5 had been inserted in an upstream position. We mapped the repression function of Kr to an alanine-rich amino-terminal region of the protein, as a Lac/Kr fusion protein containing only amino acids 26110 of Kr repressed transcription from a reporter promoter containing upstream lac operators. This demonstrates that the DNA-binding and repression activities of the Kr protein are distinct. These data are consistent with genetic evidence that Kr represses even-skipped6,7 and hunchback8 expression, and suggest that Kr is a negative regulator of transcription in Drosophila.
编码转录单元的蛋白质编码区和内含子区的序列保守性。
DOI：
10.1002/j.1460-2075.1986.tb04686.x
发表时间：
1986
期刊：
The EMBO journal
影响因子：
--
作者：
Kassis,JA;Poole,SJ;Wright,DK;O'Farrell,PH
通讯作者：
O'Farrell,PH
影响因子：
14.9
作者：
DELUCA, NA;SCHAFFER, PA
通讯作者：
SCHAFFER, PA