Symbiotic microbiome and metabolism profiles reveal the effects of induction by oysters on the metamorphosis of the carnivorous gastropod Rapana venosa.
Symbiotic microbiome and metabolism profiles reveal the effects of induction by oysters on the metamorphosis of the carnivorous gastropod Rapana venosa.
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DOI：
10.1016/j.csbj.2021.11.041
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发表时间：
2022
影响因子：
6
通讯作者：
Zhang T
中科院分区：
文献类型：
--
作者：
Yang MJ;Song H;Feng J;Yu ZL;Shi P;Liang J;Hu Z;Zhou C;Wang XL;Zhang T
大多数海洋软体动物都有一个浮游幼虫阶段，它们需要经历变态才能发育成成虫。变态受许多因素的影响，包括非生物因素如温度、盐度和光照，以及生物因素如食物和微生物。我们在前期的研究中发现脉红螺的变态需要幼牡蛎的诱导，而幼牡蛎是脉红螺的食物来源。静脉。然而，这种诱导的调节机制在很大程度上是未知的。在本研究中，我们评估了幼牡蛎诱导对R。静脉。将有能力的幼虫实验性地分成两个池，并且将没有幼牡蛎的扇贝壳和具有幼牡蛎的扇贝壳添加2小时和12小时，以监测关键基因表达、共生微生物群和代谢组学响应的改变。羧肽酶基因表达量增加，而纤维素酶基因表达量减少，这可能是幼贝诱导了牡蛎食性的转变。同时，神经内分泌系统中的关键基因在幼牡蛎中也发生了显著的变化。此外，还观察到共生菌群和代谢谱的巨大变化，其中许多与消化系统和神经内分泌系统有关。因此，幼鱼作为食物资源可能会诱导红腹锦鸡儿变态。通过调节神经内分泌系统，促进消化系统的发育和消化酶的变化。本研究为幼牡蛎诱导能促进红腹锦鸡的食性转变和变态提供了证据。通过调节代谢组和微生物组并进一步改变R. venosa的研究，拓展了我们对R.静脉。但是，还需要进一步的研究，以探索特定的物质诱导变态的幼牡蛎释放。
Most marine mollusks have a pelagic larval phase, and they need to undergo metamorphosis to develop into adults. Metamorphosis is affected by many factors, including abiotic factors such as temperature, salinity and illumination as well as biological factors such as food and microorganisms. In our previous study, we found that the metamorphosis of Rapana venosa requires induction by juvenile oysters, which are the food source of R. venosa. However, the regulatory mechanism of this induction is largely unknown. In the present study, we evaluated the impacts of induction by juvenile oysters on competent larvae of R. venosa. Competent larvae were experimentally divided into two pools, and scallop shells without juvenile oysters and scallop shells with juvenile oysters were added for 2 h and 12 h to monitor alterations in critical gene expression, symbiotic microbiota and metabolomic responses. The carboxypeptidase gene was increased while the cellulase gene was decreased, which may mean that the food habit transition was induced by juvenile oysters. Meanwhile, critical genes in the neuroendocrine system were also significantly altered in juvenile oysters. Furthermore, dramatic changes in the symbiotic microbiota and metabolism profiles were observed, with many of them associated with the digestive system and neuroendocrine system. In conclusion, juveniles as food resources may induce metamorphosis in R. venosa by regulating the neuroendocrine system and promoting the development of the digestive system and changes in digestive enzymes. This study may provide evidence that induction by juvenile oysters can promote food habit transition and metamorphosis in R. venosa by regulating the metabolome and microbiome and further altering the digestive and neuroendocrine systems of R. venosa, which expands our understanding of the regulatory mechanism of metamorphosis in R. venosa. However, further studies are needed to explore the specific substance inducing metamorphosis released by juvenile oysters.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
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影响因子：
4
作者：
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通讯作者：
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DOI：
10.1073/pnas.84.7.1867
发表时间：
1987-04-01
影响因子：
11.1
作者：
BAXTER, G;MORSE, DE
通讯作者：
MORSE, DE
影响因子：
29
作者：
Breton, Jonathan;Tennoune, Naouel;Fetissov, Serguei O.
通讯作者：
Fetissov, Serguei O.
太平洋牡蛎感受态幼虫和幼体的无标记蛋白质组学分析
影响因子：
3.7
作者：
Huan P;Wang H;Liu B
通讯作者：
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