Cyclic adenosine 3',5'-monophosphate negatively regulates clusterin gene expression in Leydig tumor cell lines.
Cyclic adenosine 3',5'-monophosphate negatively regulates clusterin gene expression in Leydig tumor cell lines.
复制标题
环腺苷 3,5-单磷酸负调节 Leydig 肿瘤细胞系中的簇蛋白基因表达。
作者：
O. Pignataro;Z. Feng;C. L. Chen
clusterin蛋白及其信使RNA在包括睾丸在内的多种组织中均有表达。在这份报告中，我们证明了clusterin基因的表达在四个Leydig肿瘤细胞系，包括小鼠MA-10和I-10和大鼠R2 C和LC-540。当细胞与0.1 mM 8-溴-cAMP或（Bu）2cAMP孵育17 h时，观察到簇蛋白mRNA积累的意外的、深刻的抑制。在MA-10和R2 C细胞中观察到clusterin mRNA下降60-70%，在I-10细胞中观察到10%，而在LC-540细胞中没有明显变化。cAMP的抑制作用是针对丛生蛋白基因的，因为在相同的细胞中，MA-10和I-10细胞中胆固醇侧链切割酶mRNA急剧升高，而所有四种细胞系中α-微管蛋白mRNA水平没有变化。早在4小时就可以检测到减少，并且在cAMP给药后17小时明显。在17 h去除培养基中的cAMP阻止了clusterin mRNA的下降。丛生蛋白基因表达的抑制也可以通过用人CG或毛喉素处理来证明，已知其升高细胞内cAMP水平。我们的观察表明：cAMP对clusterin基因表达的抑制作用可能是通过蛋白激酶A途径实现的; cAMP对clusterin基因表达的抑制作用在4种Leydig肿瘤细胞系中存在差异。
The clusterin protein and its messenger RNA were identified in many tissues including testis. In this report, we demonstrate the expression of clusterin gene in four Leydig tumor cell lines, including mouse MA-10 and I-10 and rat R2C and LC-540. When the cells were incubated with 0.1 mM 8-bromo-cAMP or (Bu)2cAMP for 17 h, an unexpected, profound suppression of clusterin mRNA accumulation was observed. A 60-70% decrease in clusterin mRNA was observed in MA-10 and R2C cells, 10% in I-10 cells, and no apparent change in LC-540 cells. The inhibitory effect of cAMP was specific to the clusterin gene, since in the same cells cholesterol side-chain cleavage enzyme mRNA was drastically elevated in MA-10 and I-10 cells while alpha-tubulin mRNA levels were not changed in all four cell lines. The reduction could be detected as early as 4 h, and was evident at 17 h after cAMP administration. Removal of cAMP from culture media at 17 h prevented the decline of clusterin mRNA. The suppression of clusterin gene expression can also be demonstrated by treatment with human CG or forskolin, which were known to elevate intracellular cAMP levels. Our observations suggest: 1) cAMP negatively regulates clusterin gene expression in two Leydig tumor cell lines, MA-10 and R2C; 2) The inhibitory effect of cAMP on clusterin gene expression is probably acting through the protein kinase A pathway; and 3) The four Leydig tumor cell lines respond differently to cAMP in the expression of clusterin and side-chain cleavage genes.
登录
查看更多内容
大鼠肝癌细胞中纤溶酶原激活剂和纤溶酶原激活剂抑制剂信使 RNA 的环核苷酸调节。
DOI：
10.1210/mend-4-1-171
发表时间：
1990
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Heaton,JH;Gelehrter,TD
通讯作者：
Gelehrter,TD
DOI：
--
发表时间：
1988-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Deutsch;J. Hoeffler;J. Jameson;J. Lin;J. Habener
通讯作者：
P. Deutsch;J. Hoeffler;J. Jameson;J. Lin;J. Habener
DOI：
10.1016/s0006-291x(88)81099-4
发表时间：
1988-08
影响因子：
3.1
作者：
C. Cheng;C. L. Chen;Z. Feng;A. Marshall;C. Bardin
通讯作者：
C. Cheng;C. L. Chen;Z. Feng;A. Marshall;C. Bardin
影响因子：
4.1
作者：
BETTUZZI, S;HIIPAKKA, RA;LIAO, SS
通讯作者：
LIAO, SS
在环磷酸腺苷促进白血病细胞分化过程中，c-myc 表达快速转录下调。
DOI：
10.1172/jci112987
发表时间：
1987
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Slungaard,A;Confer,DL;Schubach,WH
通讯作者：
Schubach,WH