Coordination of root auxin with the fungus Piriformospora indica and bacterium Bacillus cereus enhances rice rhizosheath formation under soil drying
Coordination of root auxin with the fungus Piriformospora indica and bacterium Bacillus cereus enhances rice rhizosheath formation under soil drying
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根部生长素与真菌 Piriformospora indica 和细菌 Bacillus cereus 的协调增强了土壤干燥条件下水稻根鞘的形成
DOI：
10.1038/s41396-021-01133-3
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发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Xu WF
中科院分区：
文献类型：
--
作者：
Xu F;Liao H;Zhang Y;Yao M;Liu J;Sun L;Zhang X;Yang J;Wang K;Wang X;Ding Y;Liu C;Rensing C;Zhang J;Yeh K;Xu WF
适度土壤干燥是一项具有广阔应用前景的农业技术，它可以减少水分消耗，促进根鞘形成，提高植物的抗旱性。内生真菌梨形孢菌（Piriformospora indica，P. indica）生长素产量高，可能有利于根鞘的形成。然而，P. indica与原生土壤微生物组在根鞘形成中的综合作用尚不清楚。本研究利用高通量测序技术和水稻突变体研究了稻瘟病菌和原生细菌对MSD条件下水稻根鞘形成的作用。在MSD下，接种P.indica使水稻根鞘形成显著增加约30%。水稻根和印度稻根鞘的形成主要是由于水稻根和印度稻根鞘中的甜菜碱。其次，在MSD条件下，接种印度稻后，水稻根鞘和根际中芽孢杆菌属（被称为植物根际促生细菌）的丰度得到富集。此外，蜡状芽孢杆菌（B. cereus）的生长素产量进一步增加。在接种P.indica和B.在蜡状芽孢杆菌中，野生型或生长素外排载体OsPIN2互补系水稻的根鞘形成率高于OsPIN2突变体。总之，我们的研究结果表明，内生真菌P.indica与本地土壤细菌B。蜡状芽孢杆菌通过生长素调节水稻和微生物生长促进水稻根鞘形成。这一发现揭示了印度稻和本地微生物群在适度土壤干燥条件下水稻根鞘形成中的合作贡献，这对于改善农业用水具有重要意义。
Moderate soil drying (MSD) is a promising agricultural technique that can reduce water consumption and enhance rhizosheath formation promoting drought resistance in plants. The endophytic fungus Piriformospora indica (P. indica) with high auxin production may be beneficial for rhizosheath formation. However, the integrated role of P. indica with native soil microbiome in rhizosheath formation is unclear. Here, we investigated the roles of P. indica and native bacteria on rice rhizosheath formation under MSD using high-throughput sequencing and rice mutants. Under MSD, rice rhizosheath formation was significantly increased by around 30% with P. indica inoculation. Auxins in rice roots and P. indica were responsible for the rhizosheath formation under MSD. Next, the abundance of the genus Bacillus, known as plant growth-promoting rhizobacteria, was enriched in the rice rhizosheath and root endosphere with P. indica inoculation under MSD. Moreover, the abundance of Bacillus cereus (B. cereus) with high auxin production was further increased by P. indica inoculation. After inoculation with both P. indica and B. cereus, rhizosheath formation in wild-type or auxin efflux carrier OsPIN2 complemented line rice was higher than that of the ospin2 mutant. Together, our results suggest that the interaction of the endophytic fungus P. indica with the native soil bacterium B. cereus favors rice rhizosheath formation by auxins modulation in rice and microbes under MSD. This finding reveals a cooperative contribution of P. indica and native microbiota in rice rhizosheath formation under moderate soil drying, which is important for improving water use in agriculture.
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