The K296-D320 region of recombinant levansucrase BA-SacB can affect the sensitivity of Escherichia coli host to sucrose
The K296-D320 region of recombinant levansucrase BA-SacB can affect the sensitivity of Escherichia coli host to sucrose
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重组左聚蔗糖酶BA-SacB的K296-D320区域可影响大肠杆菌宿主对蔗糖的敏感性
DOI：
10.1007/s13213-019-01496-1
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发表时间：
2019-11
影响因子：
3
通讯作者：
Yingfeng An
中科院分区：
文献类型：
--
作者：
Song Gao;Bo Jiang;Song Zhu;Darren J.Hart;Xia Liu;Hongling Wang;Yingfeng An
PurposeA mutant BA-SacB-Del encoding BA-SacB minus K296-D320 region was constructed to analyze its effect on catalytic characteristics of the enzyme as well as help deepen understanding of the catalytic mechanism of BA-SacB and even proteins of GH68 family.MethodsBased on the comparison of levansucrases from Bacillus amyloliquefaciens (BA-SacB) and Sphingopyxis macrogoltabida (SM-Lev), a mutant BA-SacB-Del encoding BA-SacB minus K296-D320 region was constructed and its effect on catalytic characteristics of the enzyme was analyzed.ResultsDeletion of this region would undoubtedly affect the conserved structure (i.e., central negatively charged cavity surrounded by five antiparallel β-strands) shared by the GH68 family. Therefore, Escherichia coli-expressing mutant BA-SacB-Del could more efficiently catalyze the production of levan in media containing high concentration of sucrose, which is unrealizable for BA-SacB.ConclusionsThis result should be valuable for understanding this conditional lethal mechanism. Therefore, this study should be very valuable for understanding the catalytic mechanism of BA-SacB and even proteins of the GH68 family. More importantly, levan can be conveniently produced by direct fermentation of sucrose-containing media with E. coli-expressing BA-SacB-Del which is not sensitive to sucrose.
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影响因子：
3.1
作者：
A. Shimamura;K. Tsuboi;T. Nagase;M. Ito;H. Tsumori;H. Mukasa
通讯作者：
A. Shimamura;K. Tsuboi;T. Nagase;M. Ito;H. Tsumori;H. Mukasa
影响因子：
5
作者：
Wenjing Li;Shuhuai Yu;Tao Zhang;B. Jiang;W. Mu
通讯作者：
Wenjing Li;Shuhuai Yu;Tao Zhang;B. Jiang;W. Mu
影响因子：
5
作者：
BAILEY, MJ
通讯作者：
BAILEY, MJ
DOI：
10.1007/978-1-4939-1720-4_2
发表时间：
2015-01-01
期刊：
BACTERIAL PANGENOMICS: METHODS AND PROTOCOLS
影响因子：
--
作者：
Dziewit, Lukasz;Bartosik, Dariusz
通讯作者：
Bartosik, Dariusz
影响因子：
4.1
作者：
J. Seo;K. Song;K. Jang;C. H. Kim;B. Jung;S. Rhee
通讯作者：
J. Seo;K. Song;K. Jang;C. H. Kim;B. Jung;S. Rhee