Argonautes promote male fertility and provide a paternal memory of germline gene expression in C. elegans.
Argonautes promote male fertility and provide a paternal memory of germline gene expression in C. elegans.
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DOI：
10.1016/j.cell.2013.11.032
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发表时间：
2013-12-19
期刊：
影响因子：
64.5
通讯作者：
Mello CC
中科院分区：
文献类型：
--
作者：
Conine CC;Moresco JJ;Gu W;Shirayama M;Conte D Jr;Yates JR 3rd;Mello CC
在每个生命周期中，生殖细胞保存并传递遗传和表观遗传信息。在线虫中，ALG-3/4 ArgAerte蛋白在雄配子发生过程中表达，并促进雄性生育。在这里，我们证明了CSR-1与ALG-3/4一起发挥作用，正向调节精子发生所需的靶基因。我们的发现表明，ALG-3/4在精子发生过程中发挥作用，放大一个小RNA信号，该信号代表对男性特定基因表达的表观遗传记忆。CSR-1在成熟精子中含量丰富，似乎将这种记忆传递给后代。令人惊讶的是，除了针对男性特定基因的小RNA外，我们还发现男性还拥有大量针对卵子发生特定基因的CSR-1小RNA。综上所述，这些发现表明，线虫精子不仅传递基因组，还以ArgAerte/Small-RNA复合体的形式传递表观遗传二元信号，这些复合体构成对前几代基因表达的记忆。
During each life cycle germ cells preserve and pass on both genetic and epigenetic information. In C. elegans, the ALG-3/4 Argonaute proteins are expressed during male gametogenesis and promote male fertility. Here we show that the CSR-1 Argonaute functions with ALG-3/4 to positively regulate target genes required for spermiogenesis. Our findings suggest that ALG-3/4 functions during spermatogenesis to amplify a small-RNA signal that represents an epigenetic memory of male-specific gene expression. CSR-1, which is abundant in mature sperm, appears to transmit this memory to offspring. Surprisingly, in addition to small RNAs targeting male-specific genes, we show that males also harbor an extensive repertoire of CSR-1 small RNAs targeting oogenesis-specific mRNAs. Together these findings suggest that C. elegans sperm transmit not only the genome but also epigenetic binary signals in the form of Argonaute/small-RNA complexes that constitute a memory of gene expression in preceding generations.
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影响因子：
64.5
作者：
Ashe A;Sapetschnig A;Weick EM;Mitchell J;Bagijn MP;Cording AC;Doebley AL;Goldstein LD;Lehrbach NJ;Le Pen J;Pintacuda G;Sakaguchi A;Sarkies P;Ahmed S;Miska EA
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64.5
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DOI：
10.1073/pnas.0906378106
发表时间：
2009-11-03
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
16
作者：
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通讯作者：
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