Increased long-flight activity triggered in beet armyworm by larval feeding on diet containing Cry1Ac protoxin.
Increased long-flight activity triggered in beet armyworm by larval feeding on diet containing Cry1Ac protoxin.
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甜菜夜蛾幼虫食用含有 Cry1Ac 原毒素的食物可引发长距离飞行活动增加。
DOI：
10.1371/journal.pone.0063554
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Luo LZ
中科院分区：
文献类型：
--
作者：
Jiang XF;Chen J;Zhang L;Sappington TW;Luo LZ
转基因作物的生态安全性评价和有害生物风险分析是其商业化种植前的重要内容。甜菜粘虫（Spodoptera exigua）是一种远距离迁徙的害虫，在中国并不是转基因cry1ac棉花的直接目标，但近年来已成为重要的害虫。移徙者离开其出生地，在很短的时间内到达遥远的其他适当栖息地，随后往往是幼虫爆发。葡萄球菌对Cry1Ac的敏感性较低。然而，我们的实验室实验结果表明：(1)Cry1Ac原毒素对幼虫的发育速度、幼虫和蛹的重量以及成虫一生的繁殖力有亚致死作用；(2)Cry1Ac引发的长时间飞行行为增加，这可能导致幼虫在其他地方爆发。在低浓度Cry1Ac原毒素人工饲料中，对照组和对照组的幼虫死亡率、化蛹率、成虫羽化率、寿命、产卵前期和产卵期均无显著差异。对某些发育和生殖性状的负亚致死效应和对其他性状的无影响表明，它们不是导致采食Cry1Ac棉花后所观察到的严重程度的原因。有趣的是，当幼虫在含有两种低剂量Cry1Ac的饲料中饲养时，长飞者的比例显著增加，这表明Cry1Ac可能增加了长距离分散的倾向。我们假设，饮食中Cry1Ac对发育和繁殖的负面影响被不良食物条件引发的飞行倾向增加所抵消，从而提高了在产卵前逃离不利当地条件的机会。增加的长途飞行倾向反过来又可能扩大爆发人群所破坏的区域。这种现象可能在其他接受亚致死剂量Bt毒素的迁徙害虫中也很常见，值得进一步研究。
Evaluating ecological safety and conducting pest risk analysis for transgenic crops are vitally important before their commercial planting. The beet armyworm, Spodoptera exigua, a long-distance migratory insect pest, is not a direct target of transgenic Cry1Ac-expressing cotton in China, but nevertheless it has recently become an important pest. Migrants leaving their natal field arrive in other appropriate habitat far away in a short time, often followed by larval outbreaks. S. exigua has low susceptibility to Cry1Ac. However, our results from laboratory experiments identified (i) sublethal effects of Cry1Ac protoxin on larval development rate, larval and pupal weight, and adult lifetime fecundity, and (ii) increased long-flight behavior triggered by Cry1Ac which may contribute to larval outbreaks elsewhere. No significant differences in larval mortality, pupation rate, adult emergence rate, longevity, pre-oviposition period, or oviposition period were observed between controls and larvae fed on artificial diet incorporating a low concentration of Cry1Ac protoxin. The negative sublethal effects on some developmental and reproductive traits and lack of effect on others suggest they do not contribute to the observed severity of S. exigua outbreaks after feeding on Cry1Ac cotton. Interestingly, the percentage of long fliers increased significantly when larvae were reared on diet containing either of two low-dose treatments of Cry1Ac, suggesting a possible increased propensity to disperse long distances triggered by Cry1Ac. We hypothesize that negative effects on development and reproduction caused by Cry1Ac in the diet are offset by increased flight propensity triggered by the poor food conditions, thereby improving the chances of escaping adverse local conditions before oviposition. Increased long-flight propensity in turn may amplify the area damaged by outbreak populations. This phenomenon might be common in other migratory insect pests receiving sublethal doses of Bt toxins and warrants further study.
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影响因子：
1.4
作者：
Molina-Ochoa, J;Lezama-Gutierrez, R;Arceo-Palacios, F
通讯作者：
Arceo-Palacios, F
影响因子：
2.3
作者：
Anilkumar, Konasale J.;Sivasupramaniam, Sakuntala;Moar, William J.
通讯作者：
Moar, William J.
影响因子：
1.7
作者：
ALI, A;GAYLOR, MJ
通讯作者：
GAYLOR, MJ
影响因子：
0.4
作者：
Greenberg, S. M.;Adamczyk, J. J.
通讯作者：
Adamczyk, J. J.
影响因子：
2.2
作者：
Jiang, Xing Fu;Luo, Li Zhi;Sappington, Thomas W.
通讯作者：
Sappington, Thomas W.