Identification and characterization of osteoclast-like cells and their progenitors in cultures of feline marrow mononuclear cells.
Identification and characterization of osteoclast-like cells and their progenitors in cultures of feline marrow mononuclear cells.
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DOI：
10.1083/jcb.99.2.471
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发表时间：
1984-08
期刊：
影响因子：
--
通讯作者：
Mundy GR
中科院分区：
文献类型：
--
作者：
Ibbotson KJ;Roodman GD;McManus LM;Mundy GR
负责骨吸收的主要细胞，即多核破骨细胞，由于难以接近而很难研究。当猫骨髓来源的单核细胞在长期培养中建立时，多核细胞在 48 小时内形成，并在 16 天时达到最大数量。我们观察到这些培养的细胞具有破骨细胞的许多特征。从形态上看，它们是多核的，含有大量的分支线粒体，具有缺乏细胞器（清晰区域）的外周细胞质，并且具有广泛的细胞表面突起。除了这些超微结构特征外，细胞还含有抗酒石酸酸性磷酸酶，其活性会被甲状旁腺激素 (PTH) 增强，并被降钙素抑制。 PTH、前列腺素 E2 和 1,25(OH)2 维生素 D3 增加多核细胞的形成，而降钙素则抑制 PTH 的刺激作用。延时电影显微摄影和放射自显影研究表明，多核细胞是由单核祖细胞融合形成的。多核细胞被吞噬并被非特异性酯酶染色，这与它们源自未成熟的单核细胞一致。此外，富含多核细胞的细胞群从失活的骨中释放 45Ca。 Percoll 上的密度梯度离心用于富集和表征这些多核细胞的单核祖细胞。祖细胞主要存在于 Percoll 密度层中，密度为 1.065 至 1.08 g/ml，与未分级细胞相比富集高达 30 倍。形成多核细胞的骨髓单核细胞最初不粘附于塑料，被非特异性酯酶严重染色，并且在组织学上似乎是未成熟的单核细胞。这些数据表明，我们培养物中的多核破骨细胞样细胞源自对骨激素有反应的非贴壁单核祖细胞。这些细胞在体外生长和表征的能力应有助于研究阐明这些细胞在骨吸收的正常和病理状态中所发挥的作用。
The predominant cell responsible for bone resorption, the multinucleated osteoclast, has been difficult to study because of inaccessibility. When feline marrow-derived mononuclear cells are established in long-term culture, multinucleated cells form within 48 h, reaching maximum numbers at 16 d. We have observed that these cultured cells have many of the features of osteoclasts. Morphologically, they are multinucleated, contain large numbers of branched mitochondria, have a peripheral cytoplasm lacking organelles (a clear zone), and have extensive cell-surface processes. In addition to these ultrastructural features, the cells contain a tartrate- resistant acid phosphatase, the activity of which is increased by parathyroid hormone (PTH) and inhibited by calcitonin. PTH, prostaglandin E2, and 1,25(OH)2 vitamin D3 increased multinucleated cell formation, while calcitonin inhibited the stimulatory effects of PTH. Time-lapse cinemicrographic and autoradiographic studies indicated that the multinucleated cells formed by fusion of the mononuclear progenitors. The multinucleated cells were phagocytic and stained with nonspecific esterase, consistent with their being derived from immature monocytes. Further, cell populations enriched for multinucleated cells release 45Ca from devitalized bone. Density-gradient centrifugation on Percoll was used to enrich and characterize the mononuclear progenitors of these multinucleated cells. The progenitor cells were found predominantly in Percoll density layers of 1.065 to 1.08 g/ml and were enriched up to 30-fold as compared to unfractionated cells. The bone marrow mononuclear cells that formed the multinucleated cells were initially nonadherent to plastic, stained heavily with nonspecific esterase, and appeared to be immature monocytes histologically. These data indicate that the multinucleated osteoclast-like cells in our cultures are derived from nonadherent monocytic progenitor cells that are responsive to osteotropic hormones. The ability to grow and characterize these cells in vitro should facilitate studies to elucidate the role these cells play in normal and pathologic states of bone resorption.
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影响因子：
--
作者：
OSDOBY, P;MARTINI, MC;CAPLAN, AI
通讯作者：
CAPLAN, AI
DOI：
10.1007/bf01890996
发表时间：
1972-01-01
期刊：
HISTOCHEMICAL JOURNAL
影响因子：
--
作者：
DOTY, SB;SCHOFIELD, BH
通讯作者：
SCHOFIELD, BH
影响因子：
4.8
作者：
LUBEN, RA;WONG, GL;COHN, DV
通讯作者：
COHN, DV
影响因子：
4.8
作者：
WENER, JA;RAISZ, LG;GORTON, SJ
通讯作者：
GORTON, SJ
影响因子：
64.8
作者：
DEXTER, TM;MOORE, MAS
通讯作者：
MOORE, MAS