Tick surveillance for relapsing fever spirochete Borrelia miyamotoi in Hokkaido, Japan.
Tick surveillance for relapsing fever spirochete Borrelia miyamotoi in Hokkaido, Japan.
复制标题
DOI：
10.1371/journal.pone.0104532
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kawabata H
中科院分区：
文献类型：
--
作者：
Takano A;Toyomane K;Konnai S;Ohashi K;Nakao M;Ito T;Andoh M;Maeda K;Watarai M;Sato K;Kawabata H
2012-2013年间，从日本最北端岛屿北海道的不同地区共收集到4325只属于硬蜱属的寻找宿主的成虫。用实时荧光定量聚合酶链式反应检测硬体裂解物检测疏螺旋体感染情况。该方法是为检测复发性发热螺旋体和非特异性莱姆病相关螺旋体而设计的。总感染率为2%(71/3532)、4.3%(5/117)和0.1%(1/676)。全沟伊蚊和巴甫洛夫斯基伊蚊的感染率显著高于卵形伊蚊。在所有物种中都发现了与莱姆病相关的螺旋体的混合感染。在本研究中，我们通过BSK-M培养获得了6株宫本芽孢杆菌，它们分别来自过硫杆菌和巴氏杆菌。根据3个看家基因(glpQ、16S rDNA和flb)构建的系统发育树表明，北海道分离株与俄罗斯宫本杆菌聚在一起，但与北美和欧洲的宫本杆菌不同。对8个基因(clpA、clpX、Nifs、PepX、pyrG、recG、rplB和uvrA)的多位点序列分析表明，所有日本宫本芽孢杆菌分离株，包括过去的分离株，都是基因克隆的，尽管这些分离株来自不同的扁虱和脊椎动物来源。从这些结果来看，感染宫本线虫的壁虱广泛分布在北海道各地。雌性的全沟伊蚊是大多数人类扁虱叮咬的罪魁祸首，因此，全沟伊蚊很可能是北海道扁虱叮咬引起的本土回归热的最重要的媒介。
During 2012–2013, a total of 4325 host-seeking adult ticks belonging to the genus Ixodes were collected from various localities of Hokkaido, the northernmost island of Japan. Tick lysates were subjected to real-time PCR assay to detect borrelial infection. The assay was designed for specific detection of the Relapsing fever spirochete Borrelia miyamotoi and for unspecific detection of Lyme disease-related spirochetes. Overall prevalence of B. miyamotoi was 2% (71/3532) in Ixodes persulcatus, 4.3% (5/117) in Ixodes pavlovskyi and 0.1% (1/676) in Ixodes ovatus. The prevalence in I. persulcatus and I. pavlovskyi ticks were significantly higher than in I. ovatus. Co-infections with Lyme disease-related spirochetes were found in all of the tick species. During this investigation, we obtained 6 isolates of B. miyamotoi from I. persulcatus and I. pavlovskyi by culture in BSK-M medium. Phylogenetic trees of B. miyamotoi inferred from each of 3 housekeeping genes (glpQ, 16S rDNA, and flaB) demonstrated that the Hokkaido isolates were clustered with Russian B. miyamotoi, but were distinguishable from North American and European B. miyamotoi. A multilocus sequence analysis using 8 genes (clpA, clpX, nifS, pepX, pyrG, recG, rplB, and uvrA) suggested that all Japanese B. miyamotoi isolates, including past isolates, were genetically clonal, although these were isolated from different tick and vertebrate sources. From these results, B. miyamotoi-infected ticks are widely distributed throughout Hokkaido. Female I. persulcatus are responsible for most human tick-bites, thereby I. persulcatus is likely the most important vector of indigenous relapsing fever from tick bites in Hokkaido.
登录
查看更多内容
DOI：
10.1128/genomea.00713-13
发表时间：
2013-09-12
期刊：
Genome announcements
影响因子：
--
作者：
Hue F;Ghalyanchi Langeroudi A;Barbour AG
通讯作者：
Barbour AG
影响因子：
11.8
作者：
Platonov, Alexander E.;Karan, Ludmila S.;Krause, Peter J.
通讯作者：
Krause, Peter J.
影响因子：
2.1
作者：
Scoles, Glen;Papero, Michele;Fish, Durland
通讯作者：
Fish, Durland
影响因子：
3.7
作者：
Geller J;Nazarova L;Katargina O;Järvekülg L;Fomenko N;Golovljova I
通讯作者：
Golovljova I
影响因子：
9.4
作者：
Fraenkel, CJ;Garpmo, U;Berglund, J
通讯作者：
Berglund, J