Detection and genetic characterization of relapsing fever spirochete Borrelia miyamotoi in Estonian ticks.

Detection and genetic characterization of relapsing fever spirochete Borrelia miyamotoi in Estonian ticks.
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DOI:
10.1371/journal.pone.0051914
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发表时间:
2012
期刊:
影响因子:
3.7
通讯作者:
Golovljova I
Golovljova I
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Geller J;Nazarova L;Katargina O;Järvekülg L;Fomenko N;Golovljova I

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2008 - 2010年,I。蓖麻和I.从爱沙尼亚大陆和萨雷马岛的64个地点收集全沟蜱。存在B。在2622只蜱中发现Miyamotoi,占0.9%(23/2622)。I. persulcatus和I.蓖麻蜱差异显着,分别为2.7%(15/561)和0.4%(8/2061)。流行率最高的地区是爱沙尼亚东南部的一个地区。persulcatus和I.蓖麻属同域分布,变异范围为1.4%(1/73)~2.8%(5/178)。与B合并感染。布格多费里公司还发现了群螺旋体和森林脑炎病毒。部分16SrRNA、p66和glpQ基因的遗传特征表明,爱沙尼亚序列属于两种类型的B。miyamotoi和聚类与来自欧洲和俄罗斯的欧洲部分的序列,以及与来自西伯利亚,亚洲和日本的序列,在这里分别指定为欧洲和亚洲类型。欧洲型的爱沙尼亚序列从I.亚洲型B.在同域区的两种蜱种均显示Miyamotoi。
During the years 2008–2010 I. ricinus and I. persulcatus ticks were collected from 64 sites in mainland Estonia and on the island Saaremaa. Presence of B. miyamotoi was found in 0.9% (23/2622) of ticks. The prevalence in I. persulcatus and I. ricinus ticks differed significantly, 2.7% (15/561) and 0.4% (8/2061), respectively. The highest prevalence rates were in found South-Eastern Estonia in an area of I. persulcatus and I. ricinus sympatry and varied from 1.4% (1/73) to 2.8% (5/178). Co-infections with B. burgdorferi s.l. group spirochetes and tick-borne encephalitis virus were also revealed. Genetic characterization of partial 16S rRNA, p66 and glpQ genes demonstrated that Estonian sequences belong to two types of B. miyamotoi and cluster with sequences from Europe and the European part of Russia, as well as with sequences from Siberia, Asia and Japan, here designated as European and Asian types, respectively. Estonian sequences of the European type were obtained from I. ricinus ticks only, whereas the Asian type of B. miyamotoi was shown for both tick species in the sympatric regions.
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