Simulated Microgravity Altered the Metabolism of Loureirin B and the Expression of Major Cytochrome P450 in Liver of Rats.
Simulated Microgravity Altered the Metabolism of Loureirin B and the Expression of Major Cytochrome P450 in Liver of Rats.
复制标题
模拟微重力改变大鼠肝脏中龙血素B的代谢和主要细胞色素P450的表达
DOI：
10.3389/fphar.2018.01130
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发表时间：
2018
影响因子：
5.6
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Chen B;Guo J;Wang S;Kang L;Deng Y;Li Y
Loureirin B (LB) is the marker compound of dragon blood (DB), which exhibits great potentials in protecting astronauts’ health against radiation and simulated microgravity (SM). Pharmacokinetics of LB is reported to be significantly altered by SM. Here, we investigated key metabolic features of LB in rat liver microsome (RLM) and the effects of SM on LB metabolism as well as on expression of major hepatic cytochrome P450 (CYP450) isoforms. Ten metabolites were tentatively identified based on fragmentation pathways using LC-MS/MS method and elimination kinetics of LB followed a typical Michaelis–Menten equation (Vmax was 1.32 μg/min/mg and Km was 13.33 μg/mL). CYP1A2, CYP2C11, CYP2D1, and CYP3A2 were involved in the metabolism of LB and the relative strength was: CYP3A2 > CYP2C11 > CYP2D1 > CYP1A2. Comparative studies suggested that elimination of LB in RLM was remarkably increased by 3-day and 14-day SM, and the generation of identified metabolites was affected as well. Additionally, 3-day and 14-day SM showed obvious regulatory effects on the expression of major CYP450 isoforms, which might contribute to the increased elimination of LB. The data provided supports for the application of DB as a protective agent and the reasonable use of current medications metabolized by hepatic CYP450 in space missions.
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DOI：
10.3390/molecules190710650
发表时间：
2014-07-22
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Fan JY;Yi T;Sze-To CM;Zhu L;Peng WL;Zhang YZ;Zhao ZZ;Chen HB
通讯作者：
Chen HB
影响因子：
3.9
作者：
Jones, HM;Houston, JB
通讯作者：
Houston, JB
刺五加苷B和刺五加苷E对大鼠肝微粒体细胞色素P450活性的影响
影响因子：
--
作者：
Guo S;Liu Y;Lin Z;Tai S;Yin S;Liu G
通讯作者：
Liu G
DOI：
10.1016/j.jchromb.2017.03.022
发表时间：
2017-05-01
影响因子：
3
作者：
Li, Yujuan;Zhang, Yushi;Ren, Wei
通讯作者：
Ren, Wei
影响因子：
3.9
作者：
Litzenburger, Martin;Kern, Fredy;Bernhardt, Rita
通讯作者：
Bernhardt, Rita