Roles of chicken growth hormone receptor antisense transcript in chicken muscle development and myoblast differentiation.
Roles of chicken growth hormone receptor antisense transcript in chicken muscle development and myoblast differentiation.
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鸡生长激素受体反义转录本在鸡肌肉发育和成肌细胞分化中的作用
DOI：
10.3382/ps/pez416
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发表时间：
2019-12-01
期刊：
影响因子：
4.4
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Xu HD;Li T;Wang Z;Adu-Asiamah P;Leng QY;Zheng JH;Zhao ZH;An LL;Zhang XQ;Zhang L
肌肉是家禽生产中重要的经济性状之一，其产量取决于胚胎期肌纤维数量的增加。鸡GHR基因可以双向转录，既有GHR-S又有GHR-AS。这两种转录本在序列上部分互补并相互作用。迄今为止，GHR-AS在成肌细胞分化中的作用和机制仍不清楚。在本研究中，我们不仅分析了GHR-AS在成肌细胞分化阶段的表达模式，而且阐明了GHR-AS通过GH-GHR-IGF1信号通路促进成肌细胞分化。定量PCR分析表明GHR-AS在成肌细胞分化过程中增加。亚细胞定位显示GHR-AS和GHR-S在细胞核中的表达水平高于细胞质中的表达水平。 GHR-AS过表达后MyoD和MyHC的表达以及成肌细胞分化显着增加，而伤口之间的距离减小，表明GHR-AS抑制成肌细胞迁移并促进分化。另外，GH蛋白处理后GHR-AS、IGF1和MyHC的表达增加，成肌细胞分化也增加。总之，GHR-AS可能通过GH-GHR-IGF1信号通路通过增强融合和抑制迁移来促进成肌细胞分化。
Muscle is one of the important economic traits in poultry production, and its production depends on the increased number of muscle fibers during the embryonic stage. Chicken GHR gene can transcribe in double directions, possessing not only GHR-S but also GHR-AS. The 2 kinds of transcripts are partially complementation in sequences and interact with each other. Until now, the roles and mechanisms of GHR-AS in myoblast differentiation was still unknown. In this study, we not only analyzed the GHR-AS expression patterns in myoblast differentiation phase but also clarified that GHR-AS promoted myoblast differentiation via GH-GHR-IGF1 signal pathway. Quantitative PCR analysis indicated that GHR-AS was increased during myoblast differentiation. Sub-cellular localization showed that GHR-AS and GHR-S were expressed at a higher level in the nucleus than that in the cytoplasm. The expression of MyoD and MyHC and the myoblast differentiation significantly increased after GHR-AS overexpression, while the distance between wounds decreased, suggesting that GHR-AS repressed myoblast migration and promoted differentiation. Additionally, the expression of GHR -AS, IGF1 and MyHC increased after GH protein treated, and the myoblast differentiation also increased. In conclusion, GHR-AS promoted myoblast differentiation by enhancing fusion and inhibiting migration possibly via GH-GHR-IGF1 signal pathway.
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影响因子：
16
作者：
Legnini, Ivano;Morlando, Mariangela;Mangiavacchi, Arianna;Fatica, Alessandro;Bozzoni, Irene
通讯作者：
Bozzoni, Irene
Let-7b调控缺失型矮鸡生长激素受体基因的表达
影响因子：
4.4
作者：
Lin S;Li H;Mu H;Luo W;Li Y;Jia X;Wang S;Jia X;Nie Q;Li Y;Zhang X
通讯作者：
Zhang X
影响因子：
4.8
作者：
Frost, RA;Nystrom, GJ;Lang, CH
通讯作者：
Lang, CH
影响因子：
2.7
作者：
Marisela Ahumada-Solorzano, S.;Martinez-Moreno, Carlos G.;Aramburo, Carlos
通讯作者：
Aramburo, Carlos
DOI：
10.1360/yc-007-0989
发表时间：
2007-08-01
期刊：
Yichuan
影响因子：
--
作者：
Wu Gui-Qin;Zheng Jiang-Xia;Yang Ning
通讯作者：
Yang Ning