Selective capture of transcribed sequences in the functional gene analysis of microbial pathogens

Selective capture of transcribed sequences in the functional gene analysis of microbial pathogens
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微生物病原体功能基因分析中转录序列的选择性捕获

DOI:
10.1007/s00253-014-6190-9
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发表时间:
2014-11
影响因子:
5
通讯作者:
Ding, Chan
Ding, Chan
中科院分区:
工程技术2区
文献类型:
--
作者:
Yi, Li;Wang, Shaohui;Lu, Chengping;Ding, Chan

文献摘要

参考文献

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选择性捕获转录序列(SCOTS)是鉴定细菌在不同生物学过程(包括与宿主的致病性相互作用)中差异表达的基因的有效方法。该方法可用于阐明驱动和维持这种相互作用的分子机制。该方法是一种强大的遗传工具,通过使用少量mRNA并允许从宿主cDNA中分离细菌cDNA,克服了其他方法中发现的局限性。它已越来越多地用于发现参与细菌-宿主相互作用的基因。在这篇综述中,我们简要介绍了SCOTS方法,概述了该方法的技术进展,并重点介绍了该方法在几种微生物病原体中的应用。
Selective capture of transcribed sequences (SCOTS) is an effective method to identify bacterial genes differentially expressed during different biological processes, including pathogenic interactions with a host species. The method can be used to elucidate molecular mechanisms driving and maintaining such interactions. The method is a powerful genetic tool that overcomes limitations found in other methods, by working with small amounts of mRNA and allowing for the separation of bacterial cDNA from host cDNA. It has been increasingly used in the discovery of genes involved in the bacterium-host interaction. In this review, we briefly introduce the SCOTS method, outline the technical advances offered in the method, and focus on the method’s applications in several microbial pathogens.
DOI: 10.1371/journal.pone.0087980
发表时间: 2014
期刊: PloS one
影响因子: 3.7
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