Dysregulated expression of mRNA and SNP in pulmonary artery remodeling in ascites syndrome in broilers.
Dysregulated expression of mRNA and SNP in pulmonary artery remodeling in ascites syndrome in broilers.
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肉鸡腹水综合征中肺动脉重塑过程中mRNA和单核苷酸多态性（SNP）的表达失调
DOI：
10.1016/j.psj.2020.11.054
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发表时间：
2021-03
期刊：
影响因子：
4.4
通讯作者：
Ping Liu
中科院分区：
文献类型：
--
作者：
Cheng S;Liu X;Liu P;Li G;Guo X;Hu G;Li L;Wu C;Xu Z;Zhou Q;Jiang J;Luo S;Huang H;Ping Liu
肉鸡腹水综合征（AS），又称肺动脉高压，是在世界范围内快速生长肉鸡中观察到的一种代谢性疾病。肺动脉重构是AS发生发展的关键步骤。肺动脉mRNA和SNP在调节AS进展中的确切关系尚不清楚。本研究取AS肉鸡肺动脉组织进行病理切片和病理解剖观察。使用GATK和ANNOVAR软件进行SNP、InDel和mRNA数据分析，研究先前报道的985个基因的SNP位点（437个上调，458个下调）。病理结果显示：疾病组腹腔及心包内有大量黄色液体，腹水心脏指数及红细胞压积发生明显变化，肺动脉重构增厚。心肌切片显示肌细胞空泡变性和肌纤维断裂。此外，ALDH7A1、IRG1、GGT5、IGSF1、DHX58、USP36、TREML2、SPAG1、CD34和PLEKHA7被发现与AS进展中肺动脉重构的发病机制密切相关。综上所述，我们目前的研究进一步阐明了AS进展中肺动脉重塑的分子机制。
Broiler ascites syndrome (AS), also called pulmonary artery hypertension, is a metabolic disorder that has been observed worldwide in fast-growing broilers. Pulmonary arterial remodeling is a key step in the development of AS. The precise relationship between mRNA and SNP of the pulmonary artery in regulating AS progression remains unclear. In this study, we obtained pulmonary artery tissues from broilers with AS to perform pathologic section and pathologic anatomic observation. SNP, InDel, and mRNA data analysis were carried out using GATK and ANNOVAR software to study the SNP loci of 985 previously reported genes (437 upregulated and 458 downregulated). The pathology results showed that there was a lot of yellow fluid in the abdominal cavity and pericardium, that the ascites cardiac index and hematocrit changed significantly, and that the pulmonary artery had remodeled and become thicker in the disease group. Myocardial sections showed vacuolar degeneration of myocytes and rupture of muscle fibers. In addition, ALDH7A1, IRG1, GGT5, IGSF1, DHX58, USP36, TREML2, SPAG1, CD34, and PLEKHA7 were found to be closely associated with the pathogenesis of pulmonary artery remodeling in AS progression. Taken together, our present study further illuminates the molecular mechanism of pulmonary artery remodeling underlying AS progression.
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影响因子：
3.7
作者：
Mullany LE;Wolff RK;Herrick JS;Buas MF;Slattery ML
通讯作者：
Slattery ML
影响因子：
3.7
作者：
Qiao L;Xie L;Shi K;Zhou T;Hua Y;Liu H
通讯作者：
Liu H
DOI：
10.1111/j.1439-0442.2007.00988.x
发表时间：
2007-12-01
期刊：
JOURNAL OF VETERINARY MEDICINE SERIES A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE
影响因子：
--
作者：
Wang, J.;Qiao, J.;Wang, X.
通讯作者：
Wang, X.
猪DHX58基因的表达模式及关联分析
影响因子：
2.4
作者：
Li, X. Y.;Han, C. M.;Zhao, S. H.
通讯作者：
Zhao, S. H.
影响因子：
8
作者：
Grant, Jennifer S.;White, Kevin;MacLean, Margaret R.;Baker, Andrew H.
通讯作者：
Baker, Andrew H.